Add to Basket Bulk discount
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) antibody

Antigen

Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1)
Synonyms B23, NPM, MGC104254, Npm, NO38, MGC102162, MGC107291, B23NP, MGC108517, npm1, MGC53901, npm
Clonality Monoclonal (7H10B9)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human, Monkey
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA
2 references available
Catalog no. ABIN966704
Quantity 0.1ml  (Variants)
Price 371.25 $   Plus shipping costs $45.00
Bulk discount
Shipping to
Availability Will be delivered in 5 to 7 Business Days

Additional Information
Application Details Publications Alternatives Variants back to top

Alternative name Nucleophosmin
Gene ID 4869
Immunogen Purified recombinant fragment of human NPM expressed in E. Coli.
Isotype IgG1
Clone 7H10B9
Description Nucleophosmin (NPM), also named B23 or NO38, is a major nucleolar protein which is 20 times more abundant in tumor or proliferating cells than in normal resting cells. NPM has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication and molecular chaperoning activities. The NPM is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase.
Synonyms: B23, NPM
Molecular Weight 33kDa

Application Details
Publications Alternatives Variants back to top

Application Notes Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Purification Ascitic fluid
Preservative 0.03% sodium azide.
Storage Store at 4°C, for long term storage, store at -20°C.
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Morris, Kirstein, Valentine et al.: "Fusion of a kinase gene, ALK, to a nucleolar protein gene, NPM, in non-Hodgkin's lymphoma." in: Science (New York, N.Y.), Vol. 263, Issue 5151, pp. 1281-4, 1994 (PubMed).

Yoneda-Kato, Look, Kirstein et al.: "The t(3;5)(q25.1;q34) of myelodysplastic syndrome and acute myeloid leukemia produces a novel fusion gene, NPM-MLF1." in: Oncogene, Vol. 12, Issue 2, pp. 265-75, 1996 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1)", type "Antibodies"
Hosts Rabbit (80), Mouse (32), Goat (6)
Reactivities Human (105), Mouse (Murine) (56), Rat (Rattus) (56), Cow (Bovine) (25), Dog (Canine) (25), Rabbit (24), Pig (Porcine) (22), Horse (Equine) (18), Chicken (4), Xenopus laevis (4), Monkey (2), Cat (Feline) (1), Chimpanzee (1), Primate (1)
Applications Western Blotting (WB) (82), ELISA (53), Immunofluorescence (IF) (51), Immunohistochemistry (IHC) (39), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (20), Immunoprecipitation (IP) (14), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunocytochemistry (ICC) (2), Immunohistochemistry (Fixed) (IHC (fx)) (2), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), Biotin (3), PE,Cy5.5 (3), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)
Epitopes N-Term (11), C-Term (9), pThr199 (9), pSer4 (5), pThr95 (5), AA 81-294 (2), AA 21-38 (1), Internal Region (1), pThr234 (1), pThr234, pThr237 (1), pThr234,pThr237 (1)