Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (pThr187) antibody

Antigen: Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B)

Synonyms: KIP1, MEN4, CDKN4, MEN1B, P27KIP1, p27, Rpn4, MGC8644, Kip1, Cdki1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18e03, CDKN1B

Binding Site: pThr187

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN966733

Additional Information

Alternative name: p27Kip1

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human p27Kip1 around the phosphorylation site of threonine 187 (E-Q-TP-P-K).

Specificity: P27Kip1 (phospho-Thr187) antibody detects endogenous levels of p27Kip1 only when phosphorylated at threonine 187.

Application Details

Application Notes: WB: 1:500~1:1000.

Concentration: 100ug/100ul.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorrylation site.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: 0.02% Sodium Azide

Storage: Store at -20°C/1 year.

Research Area: Cancer, Cell Cycle

Restrictions: For Research Use only

Publications

Product: Ishida, Kitagawa, Hatakeyama et al.: "Phosphorylation at serine 10, a major phosphorylation site of p27(Kip1), increases its protein stability." in: The Journal of biological chemistry, Vol. 275, Issue 33, pp. 25146-54, 2000 (PubMed).

Fujita, Sato, Katayama et al.: "Akt-dependent phosphorylation of p27Kip1 promotes binding to 14-3-3 and cytoplasmic localization." in: The Journal of biological chemistry, Vol. 277, Issue 32, pp. 28706-13, 2002 (PubMed).

Connor, Kotchetkov, Cariou et al.: "CRM1/Ran-mediated nuclear export of p27(Kip1) involves a nuclear export signal and links p27 export and proteolysis." in: Molecular biology of the cell, Vol. 14, Issue 1, pp. 201-13, 2003 (PubMed).

Le, Claret, Lammayot et al.: "The role of cyclin-dependent kinase inhibitor p27Kip1 in anti-HER2 antibody-induced G1 cell cycle arrest and tumor growth inhibition." in: The Journal of biological chemistry, Vol. 278, Issue 26, pp. 23441-50, 2003 (PubMed).

Eguchi, Herschenhous, Kuzushita et al.: "Helicobacter pylori increases proteasome-mediated degradation of p27(kip1) in gastric epithelial cells." in: Cancer research, Vol. 63, Issue 15, pp. 4739-46, 2003 (PubMed).