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Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (pThr202) antibody

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Antigen
Synonyms erk1/2
Epitope
pThr202
(109), (107), (26), (21), (19), (18), (14), (13), (13), (13), (13), (13), (13), (12), (10), (9), (8), (7), (6), (6), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(387), (253), (235), (43), (17), (7), (5), (5), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(318), (75), (7)
Conjugate
Un-conjugated
(10), (10), (10), (9), (6), (6), (6), (6), (6), (6), (6), (6), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC)
(287), (112), (76), (66), (52), (50), (46), (28), (16), (15), (6), (4), (2), (2), (2)
Pubmed 4 references available
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Quantity 100 μg
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human p44/42 MAP Kinase around the phosphorylation site of threonine 202 (F-L-TP-E-Y).
Specificity P44/42 MAP Kinase (Phospho-Thr202) antibody detects endogenous levels of p44/42 MAP Kinase only when phosphorylated at threonine 202.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name p44/42 MAP Kinase (MAPK1/3 Antibody Abstract)
Application Notes WB: 1:500~1:1000
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Yu, Mizumoto, Tokinaga et al.: "The inhibitory effects of sevoflurane on angiotensin II- induced, p44/42 mitogen-activated protein kinase-mediated contraction of rat aortic smooth muscle." in: Anesthesia and analgesia, Vol. 101, Issue 2, pp. 315-21, table of contents, 2005 (PubMed).

Perez, Nolan: "Simultaneous measurement of multiple active kinase states using polychromatic flow cytometry." in: Nature biotechnology, Vol. 20, Issue 2, pp. 155-62, 2002 (PubMed).

Ihn, Tamaki: "Oncostatin M stimulates the growth of dermal fibroblasts via a mitogen-activated protein kinase-dependent pathway." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 4, pp. 2149-55, 2000 (PubMed).

Hannken, Schroeder, Zahner et al.: "Reactive oxygen species stimulate p44/42 mitogen-activated protein kinase and induce p27(Kip1): role in angiotensin II-mediated hypertrophy of proximal tubular cells." in: Journal of the American Society of Nephrology : JASN, Vol. 11, Issue 8, pp. 1387-97, 2000 (PubMed).

Catalog No. ABIN966750
$398.75
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