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Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (pTyr204) antibody

Details for Product No. ABIN966751, Supplier: Log in to see
Antigen
  • erk1/2
Epitope
pTyr204
104
99
26
21
17
16
14
13
13
13
13
13
12
10
9
8
7
7
6
6
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
368
246
229
44
18
7
7
6
6
5
3
3
2
1
1
1
1
1
Host
Rabbit
302
71
7
Conjugate
Un-conjugated
10
10
10
9
6
6
6
6
6
6
6
6
3
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC)
268
102
78
66
50
46
37
29
15
10
6
4
2
2
2
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human p44/42 MAP Kinase around the phosphorylation site of tyrosine 204 (T-E-YP-V-A).
Specificity P44/42 MAP Kinase (phospho-Tyr204) antibody detects endogenous levels of p44/42 MAP Kinase only when phosphorylated at tyrosine 204.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name p44/42 MAP Kinase (MAPK1/3 Antibody Abstract)
Application Notes WB: 1:500~1:1000
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Yu, Mizumoto, Tokinaga et al.: "The inhibitory effects of sevoflurane on angiotensin II- induced, p44/42 mitogen-activated protein kinase-mediated contraction of rat aortic smooth muscle." in: Anesthesia and analgesia, Vol. 101, Issue 2, pp. 315-21, table of contents, 2005 (PubMed).

Perez, Nolan: "Simultaneous measurement of multiple active kinase states using polychromatic flow cytometry." in: Nature biotechnology, Vol. 20, Issue 2, pp. 155-62, 2002 (PubMed).

Ihn, Tamaki: "Oncostatin M stimulates the growth of dermal fibroblasts via a mitogen-activated protein kinase-dependent pathway." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 4, pp. 2149-55, 2000 (PubMed).

Hannken, Schroeder, Zahner et al.: "Reactive oxygen species stimulate p44/42 mitogen-activated protein kinase and induce p27(Kip1): role in angiotensin II-mediated hypertrophy of proximal tubular cells." in: Journal of the American Society of Nephrology : JASN, Vol. 11, Issue 8, pp. 1387-97, 2000 (PubMed).