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PAK1 antibody (P21-Activated Kinase 1) (pThr212)

Details for Product anti-PAK1 Antibody No. ABIN966796, Supplier: Log in to see
Antigen
  • X-PAK1
  • XPak1
  • pak
  • pak-1
  • pakalpha
  • PAKalpha
  • AW045634
  • PAK-1
  • Paka
Alternatives
anti-Human PAK1 antibody for Immunoprecipitation
Epitope
pThr212
54
26
24
17
13
10
10
10
9
9
7
7
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
314
148
145
4
4
1
1
Host
Rabbit
284
17
15
1
Conjugate
This PAK1 antibody is un-conjugated
11
11
11
7
7
7
4
4
4
4
4
4
4
4
4
Application
Immunohistochemistry (IHC)
221
125
123
55
51
44
22
22
13
7
6
4
1
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PAK1 around the phosphorylation site of threonine 212 (P-V-TP-P-T).
Specificity PAK1 (phospho-Thr212) antibody detects endogenous levels of PAK1 only when phosphorylated at threonine 212.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Alternative Name PAK1 (PAK1 Antibody Abstract)
Research Area Cancer, Apoptosis/Necrosis, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Alexander, Yang, Hinds: "Cellular senescence requires CDK5 repression of Rac1 activity." in: Molecular and cellular biology, Vol. 24, Issue 7, pp. 2808-19, 2004 (PubMed).

Thiel, Reeder, Pfaff et al.: "Cell cycle-regulated phosphorylation of p21-activated kinase 1." in: Current biology : CB, Vol. 12, Issue 14, pp. 1227-32, 2002 (PubMed).

Rashid, Banerjee, Nikolic: "Phosphorylation of Pak1 by the p35/Cdk5 kinase affects neuronal morphology." in: The Journal of biological chemistry, Vol. 276, Issue 52, pp. 49043-52, 2001 (PubMed).