Add to Basket Bulk discount
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

P21-Activated Kinase 1 (PAK1) (pThr212) antibody

Antigen

P21-Activated Kinase 1 (PAK1)
Synonyms PAKalpha, MGC130000, MGC130001, Paka, PAK-1, AW045634, PAK1, MGC83445, MGC139676
Binding Site
Alternatives

pThr212
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
3 references available
Catalog no. ABIN966796
Quantity 100ug  (100ug/100ul.)
Price 385.00 $   Plus shipping costs $45.00
Bulk discount
Shipping to
Availability Will be delivered in 5 to 7 Business Days

Additional Information
Application Details Publications Alternatives back to top

Alternative name PAK1
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PAK1 around the phosphorylation site of threonine 212 (P-V-TP-P-T).
Specificity PAK1 (phospho-Thr212) antibody detects endogenous levels of PAK1 only when phosphorylated at threonine 212.

Application Details
Publications Alternatives back to top

Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Research Area Cancer, Apoptosis/Necrosis, Kinases/Phosphatases
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Rashid, Banerjee, Nikolic: "Phosphorylation of Pak1 by the p35/Cdk5 kinase affects neuronal morphology." in: The Journal of biological chemistry, Vol. 276, Issue 52, pp. 49043-52, 2001 (PubMed).

Thiel, Reeder, Pfaff et al.: "Cell cycle-regulated phosphorylation of p21-activated kinase 1." in: Current biology : CB, Vol. 12, Issue 14, pp. 1227-32, 2002 (PubMed).

Alexander, Yang, Hinds: "Cellular senescence requires CDK5 repression of Rac1 activity." in: Molecular and cellular biology, Vol. 24, Issue 7, pp. 2808-19, 2004 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "P21-Activated Kinase 1 (PAK1)", type "Antibodies"
Hosts Rabbit (154), Goat (4), Mouse (4)
Reactivities Human (156), Rat (Rattus) (115), Mouse (Murine) (112), Dog (Canine) (56), Cow (Bovine) (19), Horse (Equine) (18), Cat (Feline) (1), Chicken (1)
Applications Western Blotting (WB) (91), Immunofluorescence (IF) (77), ELISA (56), Immunohistochemistry (IHC) (37), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (37), Immunoprecipitation (IP) (17), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (12), Immunocytochemistry (ICC) (7), Immunoelectron Microscopy (IEM) (4), Dot Blot (Dot) (2), Dot Blot (DB) (1), Flow Cytometry (FACS) (1)
Conjugates Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), Biotin (4), Cy3 (4), Cy5 (4), Cy5.5 (4), Cy7 (4), FITC (4), Gold (4), HRP (4), PE (4), PE,Cy3 (4), PE,Cy5 (4), PE,Cy5.5 (4), PE,Cy7 (4)
Epitopes pThr212 (30), pSer144 (18), Internal Region (8), N-Term (7), AA 525-545 (6), pSer199,Internal Region (3), pThr423 (3), Thr212 (2), Thr423 (2), AA 333-545 (1), AA 484-502 (1), C-Term (1), C-Term,AA 486-514 (1), pSer199 (1), pSer20 (1), pSer204 (1)