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P21-Activated Kinase 1 (PAK1) (pThr212) antibody

Details for Product No. ABIN966796
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Antigen
Synonyms X-PAK1, XPak1, pak, pak-1, pakalpha, PAKalpha, AW045634, PAK-1, Paka
Epitope
pThr212
(37), (18), (14), (12), (12), (10), (8), (8), (6), (5), (5), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(227), (127), (126), (38), (12), (1)
Host
Rabbit
(215), (10), (5), (1)
Conjugate
Un-conjugated
(7), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3)
Application
Immunohistochemistry (IHC)
(168), (90), (87), (51), (40), (36), (19), (14), (14), (2)
Pubmed 3 references available
Quantity 100 µg
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Catalog No. ABIN966796
385.00 $
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PAK1 around the phosphorylation site of threonine 212 (P-V-TP-P-T).
Specificity PAK1 (phospho-Thr212) antibody detects endogenous levels of PAK1 only when phosphorylated at threonine 212.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Alternative Name PAK1
Research Area Cancer, Apoptosis/Necrosis, Kinases/Phosphatases
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Rashid, Banerjee, Nikolic: "Phosphorylation of Pak1 by the p35/Cdk5 kinase affects neuronal morphology." in: The Journal of biological chemistry, Vol. 276, Issue 52, pp. 49043-52, 2001 (PubMed).

Thiel, Reeder, Pfaff et al.: "Cell cycle-regulated phosphorylation of p21-activated kinase 1." in: Current biology : CB, Vol. 12, Issue 14, pp. 1227-32, 2002 (PubMed).

Alexander, Yang, Hinds: "Cellular senescence requires CDK5 repression of Rac1 activity." in: Molecular and cellular biology, Vol. 24, Issue 7, pp. 2808-19, 2004 (PubMed).

Validation Images
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