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Pan Cytokeratin antibody
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
|3 references available|
|Quantity||5ml (0.005-0.006 mg/ml.)|
|Price||178.75 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 5 to 7 Business Days|
|Immunogen||Keratin fraction isolated from cultured cell line A-431, epidermoid carcinoma of vulva.|
|Specificity||This antibody recognizes keratin polypeptides with molecular weights of 59, 58, 56, 52.5, 56.5, 54 and 45 kD, which correspond to cytokeratins 4,5,6,8,10,13 and 18.|
Recommended Positive Control: Skin, Colonic Carcinoma.
This antibody is prediluted for Immunohistochemistry use.
Staining Procedure: The prediluted antibody can be used on frozen cryostat sections as well as formalin-fixed paraffinembedded tissue sections. For paraffin-embedded tissue sections, we recommend an incubation time and temperature of 30 minutes at 37°C for this antibody, when used in conjunction with immunoperoxidase staining kit. Prolonged fixation in buffered formalin can destroy the epitope.
|Storage||Refrigerate at 4°C. Do not freeze.|
|Restrictions||For Research Use only|
Bártek, Vojtĕsek, Stasková et al.: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991 (PubMed).
Kovarík, Rejthar, Lauerová et al.: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, 1989 (PubMed).
Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).