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Pan Cytokeratin antibody

Details for Product No. ABIN966801, Supplier: Login to see
Antigen
Reactivity
Human
55
37
34
13
9
7
7
7
7
Host
Mouse
29
28
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
3
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
22
15
13
11
10
8
8
2
2
1
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Immunogen Keratin fraction isolated from cultured cell line A-431, epidermoid carcinoma of vulva.
Clone B311-1
Isotype IgG1
Specificity This antibody recognizes keratin polypeptides with molecular weights of 59, 58, 56, 52.5, 56.5, 54 and 45 kD, which correspond to cytokeratins 4,5,6,8,10,13 and 18.
Purification Prediluted.
Pathways
Application Notes This antibody is prediluted for Immunohistochemistry use.
Staining Procedure: The prediluted antibody can be used on frozen cryostat sections as well as formalin-fixed paraffinembedded tissue sections. For paraffin-embedded tissue sections, we recommend an incubation time and temperature of 30 minutes at 37°C for this antibody, when used in conjunction with immunoperoxidase staining kit. Prolonged fixation in buffered formalin can destroy the epitope.
Comment

Recommended Positive Control: Skin, Colonic Carcinoma.

Restrictions For Research Use only
Format Liquid
Concentration 0.005-0.006 mg/ml.
Storage 4 °C
Product cited in: Kovarík, Rejthar, Lauerová et al.: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, 1989 (PubMed).

Background publications Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

Bártek, Vojt?sek, Stasková et al.: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991 (PubMed).