CD43 antibody
| Antigen |
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| Clonality |
Monoclonal (DFT1) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. |
ABIN96681 |
| Quantity |
6ml (1 to 4 ug/ml) |
| Price |
171.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Immunogen
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BALB/C mice were injected with myelomablast cell line KG1.
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Format
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Ig-fraction (prediluted)
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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DFT1
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Specificity
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This antibody reacts with an antigen expressed by normal and neoplastic T cells and myeloid lineage cells. It strongly stains T cells in the interfollicular areas of normal lymphoid tissues.
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Application Notes
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Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Tonsil. Cellular localization: Cell membrane
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Concentration
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1 to 4 ug/ml
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Buffer
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This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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CD Antigens
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Publications
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Stross, Warnke, Flavell et al.: "Molecule detected in formalin fixed tissue by antibodies MT1, DF-T1, and L60 (Leu-22) corresponds to CD43 antigen." in: Journal of clinical pathology, Vol. 42, Issue 9, pp. 953-61, 1989 (PubMed).
Borche, Lozano, Vilella et al.: "CD43 monoclonal antibodies recognize the large sialoglycoprotein of human leukocytes." in: European journal of immunology, Vol. 17, Issue 10, pp. 1523-6, 1987 (PubMed).
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Alternatives for antigen "CD43", type "Antibodies"
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Hosts
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Mouse (30), Rat (1)
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Reactivities
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Human (24), Rat (Rattus) (5), Mouse (Murine) (1)
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Applications
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Flow Cytometry (FACS) (24), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Immunoprecipitation (IP) (7), Western Blotting (WB) (7), Functional Studies (Func) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Immunohistochemistry (IHC) (2)
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Conjugates
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FITC (6), Biotin (3), PE (3), APC (2), RPE (2), Alexa Fluor 488 (1), Alexa Fluor 700 (1), Pacific Blue (1), SPRD (1)
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