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Details for Product No. ABIN966815

Pyruvate Dehydrogenase Kinase, Isozyme 1 (PDK1) (pSer241) antibody

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Antigen
Synonyms B830012B01, D530020C15Rik, si:zfos-979f1.3
Epitope
»Alternatives pSer241
Reactivity
»Alternatives Human, Mouse (Murine), Rat (Rattus)
Host
»Alternatives Rabbit
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunohistochemistry (IHC)
Pubmed 4 references available
Catalog no. ABIN966815
Quantity 100 µg
Price
385.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 5 to 7 Business Days
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PDK1 around the phosphorylation site of serine 241 (A-N-SP-F-V).
Specificity PDK1 (phospho-Ser241) antibody detects endogenous levels of PDK1 only when phosphorylated at serine 241.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name PDK1
Research Area Signaling, Cytoskeleton, Microfilaments, Kinases/Phosphatases
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Storage -20 °C
Product cited in: Chen, Nystrom, Dong et al.: "Insulin stimulates increased catalytic activity of phosphoinositide-dependent kinase-1 by a phosphorylation-dependent mechanism." in: Biochemistry, Vol. 40, Issue 39, pp. 11851-9, 2001 (PubMed).

Sato, Fujita, Tsuruo: "Regulation of kinase activity of 3-phosphoinositide-dependent protein kinase-1 by binding to 14-3-3." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39360-7, 2002 (PubMed).

Lim, Kikani, Wick et al.: "Nuclear translocation of 3'-phosphoinositide-dependent protein kinase 1 (PDK-1): a potential regulatory mechanism for PDK-1 function." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 24, pp. 14006-11, 2003 (PubMed).

Scheid, Parsons, Woodgett: "Phosphoinositide-dependent phosphorylation of PDK1 regulates nuclear translocation." in: Molecular and cellular biology, Vol. 25, Issue 6, pp. 2347-63, 2005 (PubMed).

Alternatives for antigen "Pyruvate Dehydrogenase Kinase, Isozyme 1 (PDK1)", type "Antibodies"
Hosts (122), (22), (6)
Reactivities (149), (81), (73), (28), (27), (27), (14), (12), (7), (3), (1), (1), (1), (1), (1)
Applications (115), (63), (37), (20), (15), (10), (6), (4), (4), (3), (2), (1)
Conjugates (6), (6), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (23), (11), (9), (6), (5), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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