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Pyruvate Dehydrogenase Kinase, Isozyme 1 (PDK1) (pSer241) antibody

Antigen

Pyruvate Dehydrogenase Kinase, Isozyme 1 (PDK1)
Synonyms MGC108625, B830012B01, D530020C15Rik, PDK1
Binding Site
Alternatives

pSer241
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
4 references available
Catalog no. ABIN966815
Quantity 100ug  (100ug/100ul)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name PDK1
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PDK1 around the phosphorylation site of serine 241 (A-N-SP-F-V).
Specificity PDK1 (phospho-Ser241) antibody detects endogenous levels of PDK1 only when phosphorylated at serine 241.

Application Details
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Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Research Area Signaling, Cytoskeleton, Microfilaments, Kinases/Phosphatases
Restrictions For Research Use only

Publications
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Product Chen, Nystrom, Dong et al.: "Insulin stimulates increased catalytic activity of phosphoinositide-dependent kinase-1 by a phosphorylation-dependent mechanism." in: Biochemistry, Vol. 40, Issue 39, pp. 11851-9, 2001 (PubMed).

Sato, Fujita, Tsuruo: "Regulation of kinase activity of 3-phosphoinositide-dependent protein kinase-1 by binding to 14-3-3." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39360-7, 2002 (PubMed).

Lim, Kikani, Wick et al.: "Nuclear translocation of 3'-phosphoinositide-dependent protein kinase 1 (PDK-1): a potential regulatory mechanism for PDK-1 function." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 24, pp. 14006-11, 2003 (PubMed).

Scheid, Parsons, Woodgett: "Phosphoinositide-dependent phosphorylation of PDK1 regulates nuclear translocation." in: Molecular and cellular biology, Vol. 25, Issue 6, pp. 2347-63, 2005 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Pyruvate Dehydrogenase Kinase, Isozyme 1 (PDK1)", type "Antibodies"
Hosts Rabbit (97), Mouse (10), Goat (5)
Reactivities Human (110), Rat (Rattus) (77), Mouse (Murine) (73), Cow (Bovine) (32), Chicken (31), Pig (Porcine) (29), Dog (Canine) (27), Horse (Equine) (19), Monkey (3), Cat (Feline) (2), Xenopus laevis (1)
Applications Immunofluorescence (IF) (60), Western Blotting (WB) (58), ELISA (38), Immunohistochemistry (IHC) (30), Immunoprecipitation (IP) (8), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Flow Cytometry (FACS) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (7), Alexa Fluor 488 (7), Alexa Fluor 555 (7), Alexa Fluor 647 (7), PE,Cy5.5 (7), Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pSer241 (11), pTyr9 (6), pSer396 (5), pSer410 (5), C-Term (4), pSer393 (4), pTyr376,pTyr373 (4), Internal Region (3), N-Term (3), Ser241 (2), AA 29-436 (1), AA 421-436 (1), pThr202,pTyr204 (1), pThr212 (1), pThr9 (1)