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PDK1 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 1) (pSer241)

Details for Product anti-PDK1 Antibody No. ABIN966815, Supplier: Log in to see
Antigen
  • B830012B01
  • D530020C15Rik
  • si:zfos-979f1.3
Alternatives
anti-Human PDK1 antibody for Western Blotting
Epitope
pSer241
20
16
16
9
7
6
5
5
5
3
3
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
146
40
38
6
4
4
3
3
1
1
1
Host
Rabbit
116
21
14
Conjugate
This PDK1 antibody is un-conjugated
6
5
4
3
3
3
2
2
2
1
Application
Immunohistochemistry (IHC)
123
76
40
13
8
5
5
4
3
2
1
1
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PDK1 around the phosphorylation site of serine 241 (A-N-SP-F-V).
Specificity PDK1 (phospho-Ser241) antibody detects endogenous levels of PDK1 only when phosphorylated at serine 241.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name PDK1 (PDK1 Antibody Abstract)
Research Area Signaling, Cytoskeleton, Microfilaments, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, RTK Signaling
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Scheid, Parsons, Woodgett: "Phosphoinositide-dependent phosphorylation of PDK1 regulates nuclear translocation." in: Molecular and cellular biology, Vol. 25, Issue 6, pp. 2347-63, 2005 (PubMed).

Lim, Kikani, Wick et al.: "Nuclear translocation of 3'-phosphoinositide-dependent protein kinase 1 (PDK-1): a potential regulatory mechanism for PDK-1 function." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 24, pp. 14006-11, 2003 (PubMed).

Sato, Fujita, Tsuruo: "Regulation of kinase activity of 3-phosphoinositide-dependent protein kinase-1 by binding to 14-3-3." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39360-7, 2002 (PubMed).

Chen, Nystrom, Dong et al.: "Insulin stimulates increased catalytic activity of phosphoinositide-dependent kinase-1 by a phosphorylation-dependent mechanism." in: Biochemistry, Vol. 40, Issue 39, pp. 11851-9, 2001 (PubMed).