PKC beta antibody

Catalog No. ABIN966836

Product Details anti-PKC beta Antibody

Target: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC beta antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Specificity: PKC (Ab-641) antibody detects endogenous levels of total PKC protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human PKC around the phosphorylation site of threonine 641 (E-L-TP-P-T).

Application Details

Application Notes: WB: 1:500~1:1000
IHC: 1:50~1:100.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100ug/100ul.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-PKC beta antibody (ABIN966836)

Fu, Deng, Zhao, Li, Zhou, Zhang: "Distinct expression patterns of hedgehog ligands between cultured and primary colorectal cancers are associated with aberrant methylation of their promoters." in: Molecular and cellular biochemistry, Vol. 337, Issue 1-2, pp. 185-92, (2010) (PubMed).

Zhang, Liao, Dufau et al.: "Phosphatidylinositol 3-kinase/protein kinase Czeta-induced phosphorylation of Sp1 and p107 repressor release have a critical role in histone deacetylase inhibitor-mediated derepression [corrected] of ..." in: Molecular and cellular biology, Vol. 26, Issue 18, pp. 6748-61, (2006) (PubMed).

Castoria, Migliaccio, Di Domenico, Lombardi, de Falco, Varricchio, Bilancio, Barone, Auricchio: "Role of atypical protein kinase C in estradiol-triggered G1/S progression of MCF-7 cells." in: Molecular and cellular biology, Vol. 24, Issue 17, pp. 7643-53, (2004) (PubMed).

Marcil, Harbour, Houle, Naccache, Bourgoin: "Monosodium urate-crystal-stimulated phospholipase D in human neutrophils." in: The Biochemical journal, Vol. 337 ( Pt 2), pp. 185-92, (1999) (PubMed).

Bornancin, Parker: "Phosphorylation of threonine 638 critically controls the dephosphorylation and inactivation of protein kinase Calpha." in: Current biology : CB, Vol. 6, Issue 9, pp. 1114-23, (1997) (PubMed).

Target Details for PKC beta

Target: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Alternative Name: PKCbeta (PRKCB Products)

Pathways: WNT Signaling, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling