PKD/PKCmy (pSer910) antibody

Antigen: PKD/PKCmy

Binding Site: pSer910

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN966846

Additional Information

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human PKD/PKCµ around the phosphorylation site of serine 910 (R-V-SP-I-L).

Specificity: PKD/PKCµ (phospho-Ser910) antibody detects endogenous levels of PKD/PKCµ only when phosphorylated at serine 910.

Application Details

Application Notes: WB: 1:500~1:1000

Concentration: 100ug/100ul.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: 0.02% Sodium Azide

Storage: Store at -20°C/1 year.

Restrictions: For Research Use only

Publications

Product: Matthews, Rozengurt, Cantrell: "Characterization of serine 916 as an in vivo autophosphorylation site for protein kinase D/Protein kinase Cmu." in: The Journal of biological chemistry, Vol. 274, Issue 37, pp. 26543-9, 1999 (PubMed).