Phospholipase C, gamma 2 (PLCG2) (pTyr753) antibody

Antigen: Phospholipase C, gamma 2 (PLCG2)

Binding Site: pTyr753

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN966862

Additional Information

Alternative name: PLCgamma2

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human PLC2 around the phosphorylation site of tyrosine 753 (S-L-YP-D-V).

Specificity: PLC2 (phospho-Tyr753) antibody detects endogenous levels of PLC2 only when phosphorylated at tyrosine 753.

Application Details

Application Notes: WB: 1:500~1:1000.

Concentration: 100ug/100ul.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: 0.02% Sodium Azide

Storage: Store at -20°C/1 year.

Restrictions: For Research Use only

Publications

Product: Suzuki-Inoue, Wilde, Andrews et al.: "Glycoproteins VI and Ib-IX-V stimulate tyrosine phosphorylation of tyrosine kinase Syk and phospholipase Cgamma2 at distinct sites." in: The Biochemical journal, Vol. 378, Issue Pt 3, pp. 1023-9, 2004 (PubMed).

Humphries, Dangelmaier, Sommer et al.: "Tec kinases mediate sustained calcium influx via site-specific tyrosine phosphorylation of the phospholipase Cgamma Src homology 2-Src homology 3 linker." in: The Journal of biological chemistry, Vol. 279, Issue 36, pp. 37651-61, 2004 (PubMed).

Kim, Sekiya, Poulin et al.: "Mechanism of B-cell receptor-induced phosphorylation and activation of phospholipase C-gamma2." in: Molecular and cellular biology, Vol. 24, Issue 22, pp. 9986-99, 2004 (PubMed).