Polo-Like Kinase 1 (PLK1) (pThr210) antibody

Antigen: Polo-Like Kinase 1 (PLK1)

Synonyms: PLK, STPK13, Snk, plk, MGC52566, Plx1, PLK-1, stpk13, MGC69251, MGC133927

Binding Site: pThr210

Clonality: Polyclonal

Host: Please enquire

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN966867

Additional Information

Alternative name: PLK1

Immunogen: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to Nresidues of human PLK1(Serine/threonine-protein kinase PLK1) (Polo-like kinase 1)

Description: PLK1(Serine/threonine-protein kinase) that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. PLK1 is activated by serine and threonine phosphorylation. PLK1 interacts with CEP170 and EVI5. PLK1 interacts and phosphorylates ERCC6L. PLK1 accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase. Autophosphorylation and phosphorylation of Ser-137 are not significant events during activation of PLK1 in M phase.
Synonyms: PLK

Application Details

Research Area: Cancer, Cell Cycle, Kinases/Phosphatases

Restrictions: For Research Use only

Publications

Product: Bräuninger, Strebhardt, Rübsamen-Waigmann: "Identification and functional characterization of the human and murine polo-like kinase (Plk) promoter." in: Oncogene, Vol. 11, Issue 9, pp. 1793-800, 1995 (PubMed).

Lake, Jelinek: "Cell cycle- and terminal differentiation-associated regulation of the mouse mRNA encoding a conserved mitotic protein kinase." in: Molecular and cellular biology, Vol. 13, Issue 12, pp. 7793-801, 1994 (PubMed).

Golsteyn, Schultz, Bartek et al.: "Cell cycle analysis and chromosomal localization of human Plk1, a putative homologue of the mitotic kinases Drosophila polo and Saccharomyces cerevisiae Cdc5." in: Journal of cell science, Vol. 107 ( Pt 6), pp. 1509-17, 1994 (PubMed).

Uchiumi, Longo, Ferris: "Cell cycle regulation of the human polo-like kinase (PLK) promoter." in: The Journal of biological chemistry, Vol. 272, Issue 14, pp. 9166-74, 1997 (PubMed).

Lee, Erikson: "Plk is a functional homolog of Saccharomyces cerevisiae Cdc5, and elevated Plk activity induces multiple septation structures." in: Molecular and cellular biology, Vol. 17, Issue 6, pp. 3408-17, 1997 (PubMed).

Jang, Ma, Terada et al.: "Phosphorylation of threonine 210 and the role of serine 137 in the regulation of mammalian polo-like kinase." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 44115-20, 2002 (PubMed).

Wind, Kelm, Nigg et al.: "Identification of phosphorylation sites in the polo-like kinases Plx1 and Plk1 by a novel strategy based on element and electrospray high resolution mass spectrometry." in: Proteomics, Vol. 2, Issue 11, pp. 1516-23, 2002 (PubMed).