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PPARG antibody (Peroxisome Proliferator-Activated Receptor gamma) (N-Term)

Details for Product anti-PPARG Antibody No. ABIN966886, Supplier: Log in to see
Antigen
  • PPAR gamma
  • PPARg2
  • PPARG
  • Nr1c3
  • PPAR-gamma
  • PPAR-gamma2
  • PPARgamma
  • PPARgamma2
  • NR1C3
  • CIMT1
  • GLM1
  • PPARG1
  • PPARG2
  • xPPAR-gamma
  • PPAR-GAMMA
  • PPARGAMMA
  • peroxisome proliferator-activated receptor gamma
  • peroxisome proliferator activated receptor gamma
  • PPAR gamma 3
  • PPARG
  • Pparg
  • pparg
  • LOC100008892
Alternatives
anti-Human PPARG antibody for ELISA
Epitope
N-Term
39
25
18
17
16
15
15
11
11
9
7
5
5
5
5
5
5
5
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
271
153
117
31
22
17
12
11
11
8
6
6
5
5
4
4
3
3
2
2
2
2
1
1
1
Host
Rabbit
257
33
5
5
Clonality
Polyclonal
Conjugate
This PPARG antibody is un-conjugated
18
15
9
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
Application
Western Blotting (WB)
241
130
62
42
39
30
20
14
13
10
8
6
3
3
2
2
Options
Supplier
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Immunogen A synthetic peptide corresponding to a sequence at the N-terminal of human PPAR-G, different from the rat and mouse sequences by two amino acids.< br/>Immunogen was affinity purified.
Isotype IgG
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Peroxisome proliferator-activated receptor gamma ( PPARG) detection. Tested with WB in Human.
Background The peroxisome proliferator-activated receptors (PPARs) are a group of three nuclear receptor isoforms, PPAR gamma, PPAR alpha, and PPAR delta, encoded by different genes. PPARs are ligand-regulated transcription factors that control gene expression by binding to specific response elements (PPREs) within promoters. PPAR gamma is a transcription factor that has a pivotal role in adipocyte differentiation and expression of adipocyte-specific genes. The PPAR gamma1 and gamma2 isoforms result from alternative splicing and have ligand-dependent and -independent activation domains. PPAR gamma is a member of a family of nuclear receptors/ligand-dependent transcription factors, which bind to hormone response elements on target gene promoters. Ameshima et al. (2003) found that PPAR gamma is abundantly expressed in normal lung tissues, especially in endothelial cells, but that its expression is reduced or absent in the angiogenic plexiform lesions of pulmonary hypertensive lungs and in the vascular lesions of a rat model of severe pulmonary hypertension. And they conclude that fluid shear stress decreases the expression of PPARgamma in endothelial cells and that loss of PPARgamma expression characterizes an abnormal, proliferating, apoptosis-resistant endothelial cell phenotype.
Pathways MAPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Sodium azide, Thimerosal (Merthiolate)
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.
Product cited in: Ameshima, Golpon, Cool, Chan, Vandivier, Gardai, Wick, Nemenoff, Geraci, Voelkel: "Peroxisome proliferator-activated receptor gamma (PPARgamma) expression is decreased in pulmonary hypertension and affects endothelial cell growth." in: Circulation research, Vol. 92, Issue 10, pp. 1162-9, 2003 (PubMed).

Berger, Moller: "The mechanisms of action of PPARs." in: Annual review of medicine, Vol. 53, pp. 409-35, 2002 (PubMed).

Deeb, Fajas, Nemoto, Pihlajamäki, Mykkänen, Kuusisto, Laakso, Fujimoto, Auwerx: "A Pro12Ala substitution in PPARgamma2 associated with decreased receptor activity, lower body mass index and improved insulin sensitivity." in: Nature genetics, Vol. 20, Issue 3, pp. 284-7, 1998 (PubMed).