Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) (pSer2056) antibody

Antigen: Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC)

Synonyms: HYRC, p350, DNAPK, DNPK1, HYRC1, XRCC7, DNA-PKcs, prkdc-A, dna-pkcs, PRKDC, DNA-PKCS

Binding Site: pSer2056

Clonality: Polyclonal

Host: Please enquire

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN966894

Additional Information

Alternative name: PRKDC

Immunogen: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to middle residues of human PRKDC(DNA-dependent protein kinase catalytic subunit)

Description: PRKDC(DNA-dependent protein kinase catalytic subunit) is a Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. PRKDC must be bound to DNA to express its catalytic properties. PRKDC promotes processing of hairpin DNA structures in V(D)Jrecombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. PRKDC is required to protect and align broken ends of DNA. PRKDC may also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. PRKDC found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. PRKDC also involved in modulation of transcription. PRKDC recognizes the substrate consensus sequence [ST]-Q. PRKDC phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. PRKDC phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, c-myc/MYC and RFA2. PRKDC can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate TP53/p53 in the presence of supercoiled DNA is dependent on C1D.

Application Details

Restrictions: For Research Use only

Publications

Product: Iijima, Teraoka, Date et al.: "DNA-activated protein kinase in Raji Burkitt's lymphoma cells. Phosphorylation of c-Myc oncoprotein." in: European journal of biochemistry / FEBS, Vol. 206, Issue 2, pp. 595-603, 1992 (PubMed).

Carter, Vancurová, Sun et al.: "A DNA-activated protein kinase from HeLa cell nuclei." in: Molecular and cellular biology, Vol. 10, Issue 12, pp. 6460-71, 1991 (PubMed).

Lees-Miller, Anderson: "The human double-stranded DNA-activated protein kinase phosphorylates the 90-kDa heat-shock protein, hsp90 alpha at two NH2-terminal threonine residues." in: The Journal of biological chemistry, Vol. 264, Issue 29, pp. 17275-80, 1989 (PubMed).

Poltoratsky, Shi, York et al.: "Human DNA-activated protein kinase (DNA-PK) is homologous to phosphatidylinositol kinases." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 10, pp. 4529-33, 1995 (PubMed).

Sipley, Menninger, Hartley et al.: "Gene for the catalytic subunit of the human DNA-activated protein kinase maps to the site of the XRCC7 gene on chromosome 8." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 16, pp. 7515-9, 1995 (PubMed).

Hartley, Gell, Smith et al.: "DNA-dependent protein kinase catalytic subunit: a relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product." in: Cell, Vol. 82, Issue 5, pp. 849-56, 1995 (PubMed).

Liu, Ma, Yueh et al.: "The carboxyl-terminal transactivation domain of human serum response factor contains DNA-activated protein kinase phosphorylation sites." in: The Journal of biological chemistry, Vol. 268, Issue 28, pp. 21147-54, 1993 (PubMed).

Ladenburger, Fackelmayer, Hameister et al.: "MCM4 and PRKDC, human genes encoding proteins MCM4 and DNA-PKcs, are close neighbours located on chromosome 8q12-->q13." in: Cytogenetics and cell genetics, Vol. 77, Issue 3-4, pp. 268-70, 1997 (PubMed).