RAB25, Member RAS Oncogene Family (RAB25) antibody

Antigen: RAB25, Member RAS Oncogene Family (RAB25)

Synonyms: CATX-8, RAB11C, AV012978, MGC84182, RAB25, catx-8, MGC79587, zgc:101648, si:dkeyp-84f11.7, MGC140813

Clonality: Monoclonal

Host: Please enquire

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Catalog no.: ABIN966930

Additional Information

Alternative name: Rab25

Gene ID: 57111

Isotype: IgG2a

Description: Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50% similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa

Specificity: Ni-NTA purified recombinant Rab25 expressed in E. Coli strain BL21 (DE3)

Application Details

Application Notes: Western Blot: 1: 500- 1: 2,000
IHC(P): 1: 500- 1: 2,000
IHC(F): 1: 500- 1: 2,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.

Purification: Antibodies are purified by protein A affinity chromatography

Buffer: PBS with 0.2%BSA

Preservative: 0.09% sodium azide

Storage: Store at -20°C.

Research Area: Cancer

Restrictions: For Research Use only

Publications

Product: Goldenring, Shen, Vaughan et al.: "Identification of a small GTP-binding protein, Rab25, expressed in the gastrointestinal mucosa, kidney, and lung." in: The Journal of biological chemistry, Vol. 268, Issue 25, pp. 18419-22, 1993 (PubMed).