RAB25, Member RAS Oncogene Family (RAB25) antibody

Details for Product No. ABIN966930
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Antigen
Synonyms MGC84182, RAB25, catx-8, MGC79587, rab25, si:dkeyp-84f11.7, zgc:101648, CATX-8, RAB11C, AV012978
Reactivity
Human
(44), (20), (13), (13), (13), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host
(25), (17), (3)
Clonality
Monoclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), ELISA
(36), (17), (14), (13), (10), (7), (5), (3), (3), (2), (1), (1)
Pubmed 1 reference available
Quantity 0.1 mg
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Catalog No. ABIN966930
550.00 $
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Isotype IgG2a
Specificity Ni-NTA purified recombinant Rab25 expressed in E. Coli strain BL21 (DE3)
Purification Antibodies are purified by protein A affinity chromatography
Alternative Name Rab25
Background Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50% similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa
Gene ID 57111
Research Area Cancer
Application Notes Western Blot: 1: 500- 1: 2,000
IHC(P): 1: 500- 1: 2,000
IHC(F): 1: 500- 1: 2,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Buffer PBS with 0.2%BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Goldenring, Shen, Vaughan et al.: "Identification of a small GTP-binding protein, Rab25, expressed in the gastrointestinal mucosa, kidney, and lung." in: The Journal of biological chemistry, Vol. 268, Issue 25, pp. 18419-22, 1993 (PubMed).

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