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RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (pSer259)

Details for Product anti-RAF1 Antibody No. ABIN966940, Supplier: Log in to see
Antigen
  • c-raf
  • craf
  • raf1
  • RAF1
  • raf
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • AFR1
  • RAF
  • Afr-1
  • Afr1
  • Raf
  • mKIAA0854
  • v-raf-1 murine leukemia viral oncogene homolog 1a
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • raf protein
  • v-raf-leukemia viral oncogene 1
  • zinc fingers and homeoboxes 2
  • raf1a
  • RAF1
  • raf1
  • LOC100341921
  • LOC100347407
  • c-raf
  • Raf1
  • ZHX2
  • Zhx2
Alternatives
anti-Human RAF1 antibody for Enzyme Immunoassay
Epitope
pSer259
75
70
49
39
37
36
29
29
28
28
23
23
22
21
20
19
17
15
15
15
15
15
14
14
11
9
9
8
8
6
6
6
6
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
819
425
401
26
25
17
16
13
9
7
7
5
4
3
2
2
1
1
1
1
Host
Rabbit
783
41
14
7
2
Conjugate
This RAF1 antibody is un-conjugated
44
44
43
29
29
29
13
13
12
12
12
12
12
12
12
12
12
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC)
494
365
215
182
156
90
52
34
19
18
17
7
2
2
2
1
Options
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Raf-1 around the phosphorylation site of serine 259 (S-T-SP-T-P).
Specificity Raf-1 (phospho-Ser259) antibody detects endogenous levels of Raf-1 only when phosphorylated at serine 259.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name Raf1 (RAF1 Antibody Abstract)
Research Area Cancer, Apoptosis/Necrosis, Signaling, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Gabriel, Bravery, Dougherty, Kayley, Malster, Scales: "Vascular access: indications and implications for patient care." in: Nursing standard (Royal College of Nursing (Great Britain) : 1987), Vol. 19, Issue 26, pp. 45-52, 2005 (PubMed).

Dougherty, Müller, Ritt, Zhou, Zhou, Copeland, Conrads, Veenstra, Lu, Morrison: "Regulation of Raf-1 by direct feedback phosphorylation." in: Molecular cell, Vol. 17, Issue 2, pp. 215-24, 2005 (PubMed).

Hekman, Fischer, Wennogle, Wang, Campbell, Rapp: "Novel C-Raf phosphorylation sites: serine 296 and 301 participate in Raf regulation." in: FEBS letters, Vol. 579, Issue 2, pp. 464-8, 2005 (PubMed).

Chong, Lee, Guan: "Positive and negative regulation of Raf kinase activity and function by phosphorylation." in: The EMBO journal, Vol. 20, Issue 14, pp. 3716-27, 2001 (PubMed).

King, Sun, Diaz, Barnard, Miao, Bagrodia, Marshall: "The protein kinase Pak3 positively regulates Raf-1 activity through phosphorylation of serine 338." in: Nature, Vol. 396, Issue 6707, pp. 180-3, 1998 (PubMed).