V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pSer259) antibody

Details for Product No. ABIN966940
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Antigen
Synonyms NS5, CRAF, Raf-1, c-Raf, 6430402F14Rik, AA990557, BB129353, Craf1, D830050J10Rik, v-Raf, raf, raf1, c-raf, craf, RAF1
Epitope
pSer259
(63), (44), (30), (24), (24), (24), (24), (22), (19), (19), (18), (17), (17), (17), (17), (16), (16), (6), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(513), (333), (326), (152), (149), (120), (113), (12), (2), (2)
Host
Rabbit
(470), (33), (15), (8), (1)
Conjugate
Un-conjugated
(13), (13), (13), (13), (13), (13), (13), (13), (13), (13), (13)
Application
Immunohistochemistry (IHC)
(337), (211), (130), (109), (96), (41), (27), (11), (10), (10), (2), (2), (1), (1), (1)
Pubmed 5 references available
Catalog no. ABIN966940
Quantity 100 µg
Price
385.00 $   Plus shipping costs $45.00
Options
Shipping to United States (Change)
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Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Raf-1 around the phosphorylation site of serine 259 (S-T-SP-T-P).
Specificity Raf-1 (phospho-Ser259) antibody detects endogenous levels of Raf-1 only when phosphorylated at serine 259.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name Raf1
Research Area Cancer, Apoptosis/Necrosis, Signaling, Kinases/Phosphatases
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: King, Sun, Diaz et al.: "The protein kinase Pak3 positively regulates Raf-1 activity through phosphorylation of serine 338." in: Nature, Vol. 396, Issue 6707, pp. 180-3, 1998 (PubMed).

Chong, Lee, Guan: "Positive and negative regulation of Raf kinase activity and function by phosphorylation." in: The EMBO journal, Vol. 20, Issue 14, pp. 3716-27, 2001 (PubMed).

Hekman, Fischer, Wennogle et al.: "Novel C-Raf phosphorylation sites: serine 296 and 301 participate in Raf regulation." in: FEBS letters, Vol. 579, Issue 2, pp. 464-8, 2005 (PubMed).

Dougherty, Müller, Ritt et al.: "Regulation of Raf-1 by direct feedback phosphorylation." in: Molecular cell, Vol. 17, Issue 2, pp. 215-24, 2005 (PubMed).

Gabriel, Bravery, Dougherty et al.: "Vascular access: indications and implications for patient care." in: Nursing standard (Royal College of Nursing (Great Britain) : 1987), Vol. 19, Issue 26, pp. 45-52, 2005 (PubMed).

Hosts (470), (33), (15), (8), (1)
Reactivities (513), (333), (326), (152), (149), (120), (113), (12), (2), (2)
Applications (337), (211), (130), (109), (96), (41), (27), (11), (10), (10), (2), (2), (1), (1), (1)
Conjugates (13), (13), (13), (13), (13), (13), (13), (13), (13), (13), (13)
Epitopes (63), (44), (30), (24), (24), (24), (24), (22), (19), (19), (18), (17), (17), (17), (17), (16), (16), (6), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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