Retinoblastoma 1 antibody (RB1) (pSer795)

Details for Product anti-RB1 Antibody No. ABIN966951, Supplier: Log in to see
Antigen
  • RB1
  • OSRC
  • RB
  • p105-Rb
  • pRb
  • pp110
  • Rb
  • Rb-1
  • zgc:154147
  • P105-RB
  • PP110
  • RB11
  • retinoblastoma 1 (including osteosarcoma)
  • retinoblastoma 1
  • Retinoblastoma-associated protein
  • RB1
  • rb
  • Rb1
  • rb1
Alternatives
anti-Human Retinoblastoma 1 antibody for Immunofluorescence (fixed cells)
Epitope
pSer795
53
52
52
41
36
29
29
20
19
18
15
15
14
14
8
8
8
7
7
7
6
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
625
340
235
10
9
8
8
5
5
4
4
3
3
2
1
1
Host
Rabbit
460
166
1
Conjugate
This Retinoblastoma 1 antibody is un-conjugated
28
26
26
16
14
14
13
13
7
7
7
7
7
7
7
7
7
5
5
5
5
5
5
5
5
3
2
1
1
Application
Immunohistochemistry (IHC)
386
233
171
167
94
91
49
41
28
25
10
9
7
7
6
2
1
1
1
Options
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Rb around the phosphorylation site of serine 795 (P-S-SP-P-L).
Specificity Rb (phospho-Ser795) antibody detects endogenous levels of Rb only when phosphorylated at serine 795 Tested
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name Rb (RB1 Antibody Abstract)
Research Area Cancer, Transcription Factors
Pathways Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Background publications Leibundgut, Schmitz, Hirt: "Catalytic activities of G1 cyclin-dependent kinases and phosphorylation of retinoblastoma protein in mobilized peripheral blood CD34+ hematopoietic progenitor cells." in: Stem cells (Dayton, Ohio), Vol. 23, Issue 7, pp. 1002-11, 2005 (PubMed).

Rubin, Gall, Zheng, Pavletich: "Structure of the Rb C-terminal domain bound to E2F1-DP1: a mechanism for phosphorylation-induced E2F release." in: Cell, Vol. 123, Issue 6, pp. 1093-106, 2005 (PubMed).

Wallick, Gamper, Thorne, Feith, Takasaki, Wilson, Seki, Pegg, Byus, Bachmann: "Key role for p27Kip1, retinoblastoma protein Rb, and MYCN in polyamine inhibitor-induced G1 cell cycle arrest in MYCN-amplified human neuroblastoma cells." in: Oncogene, Vol. 24, Issue 36, pp. 5606-18, 2005 (PubMed).

Product cited in: Roesch, Becker, Meyer, Hafner, Wild, Landthaler, Vogt: "Overexpression and hyperphosphorylation of retinoblastoma protein in the progression of malignant melanoma." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 18, Issue 4, pp. 565-72, 2005 (PubMed).