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Retinoblastoma 1 (RB1) (pSer807) antibody

Antigen

Retinoblastoma 1 (RB1)
Synonyms RB, pRb, OSRC, pp110, p105-Rb, Rb, Rb-1, RB1, RB11, PP110, P105-RB
Binding Site
Alternatives

pSer807
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
4 references available
Catalog no. ABIN966952
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name Rb
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Rb around the phosphorylation site of serine 807 (Y-I-SP-P-L).
Specificity Rb (phospho-Ser807) antibody detects endogenous levels of Rb only when phosphorylated at serine 807.

Application Details
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Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Research Area Cancer, Transcription Factors
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Knudsen, Wang: "Differential regulation of retinoblastoma protein function by specific Cdk phosphorylation sites." in: The Journal of biological chemistry, Vol. 271, Issue 14, pp. 8313-20, 1996 (PubMed).

Knudsen, Wang: "Dual mechanisms for the inhibition of E2F binding to RB by cyclin-dependent kinase-mediated RB phosphorylation." in: Molecular and cellular biology, Vol. 17, Issue 10, pp. 5771-83, 1997 (PubMed).

Chadee, Kyriakis: "MLK3 is required for mitogen activation of B-Raf, ERK and cell proliferation." in: Nature cell biology, Vol. 6, Issue 8, pp. 770-6, 2004 (PubMed).

Roesch, Becker, Meyer et al.: "Overexpression and hyperphosphorylation of retinoblastoma protein in the progression of malignant melanoma." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 18, Issue 4, pp. 565-72, 2005 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Retinoblastoma 1 (RB1)", type "Antibodies"
Hosts Rabbit (333), Mouse (68), Rat (1)
Reactivities Human (397), Mouse (Murine) (259), Rat (Rattus) (259), Cow (Bovine) (92), Horse (Equine) (73), Dog (Canine) (56), Pig (Porcine) (55), Chicken (31), Mink (2), Rabbit (1), Zebrafish (1)
Applications Western Blotting (WB) (204), Immunofluorescence (IF) (182), ELISA (103), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (94), Immunohistochemistry (IHC) (69), Immunoprecipitation (IP) (54), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (30), Immunocytochemistry (ICC) (12), Flow Cytometry (FACS) (10), Immunoelectron Microscopy (IEM) (10), Immunohistochemistry (Frozen Sections) (IHC (fro)) (8), Dot Blot (Dot) (5), Immunohistochemistry (Fixed) (IHC (fx)) (2), Dot Blot (DB) (1)
Conjugates Biotin (14), Alexa Fluor 350 (10), Alexa Fluor 488 (10), Alexa Fluor 555 (10), Alexa Fluor 647 (10), Cy3 (10), Cy5 (10), Cy5.5 (10), Cy7 (10), FITC (10), Gold (10), HRP (10), PE (10), PE,Cy3 (10), PE,Cy5 (10), PE,Cy5.5 (10), PE,Cy7 (10)
Epitopes pSer780 (36), pSer795 (33), pSer608 (22), pSer612 (22), pThr821 (22), pSer249 (21), pSer788 (21), pThr826 (20), pSer807,pSer811 (14), pSer807 (11), C-Term (9), AA 393-572 (5), pSer811,pSer807 (5), pSer811 (4), Internal Region (3), N-Term (3), Ser780 (3), pThr356 (3), Ser608 (2), Ser795 (2), Ser807 (2), AA 121-140 (1), AA 251-268 (1), AA 280-300 (1), AA 371-390 (1), AA 41-330 (1), AA 511-530 (1), AA 571-590 (1), AA 612-928 (1), AA 800-850 (1), AA 886-905 (1), Center (1), pSer8111 (1), pSer821 (1), pSer826 (1), pThr252,pSer249 (1), pThr373 (1)