RICTOR antibody

Catalog No. ABIN966967

Product Details anti-RICTOR Antibody

Target: RICTOR (RPTOR Independent Companion of mTOR, Complex 2 (RICTOR))

Reactivity: Human, Mouse, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This RICTOR antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human RICTOR expressed in E. Coli.

Clone: 4H5

Isotype: IgG1

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-RICTOR antibody (ABIN966967)

Martin, Masri, Bernath, Nishimura, Gera: "Hsp70 associates with Rictor and is required for mTORC2 formation and activity." in: Biochemical and biophysical research communications, Vol. 372, Issue 4, pp. 578-83, (2008) (PubMed).

Yang, Inoki, Ikenoue, Guan: "Identification of Sin1 as an essential TORC2 component required for complex formation and kinase activity." in: Genes & development, Vol. 20, Issue 20, pp. 2820-32, (2006) (PubMed).

Target Details for RICTOR

Target: RICTOR (RPTOR Independent Companion of mTOR, Complex 2 (RICTOR))

Alternative Name: RICTOR (RICTOR Products)

Synonyms: PIA antibody, mAVO3 antibody, 4921505C17Rik antibody, 6030405M08Rik antibody, AVO3 antibody, AW492497 antibody, D530039E11Rik antibody, Fyb antibody, RPTOR independent companion of MTOR complex 2 antibody, RPTOR independent companion of MTOR, complex 2 antibody, RICTOR antibody, Rictor antibody

Background: Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals . mTOR is a large protein kinase with two different complexes. One complex contains mTOR, µL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, µL, Sin1 and rictor. The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro . This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes. This complex has also been shown to phosphorylate the rapamycinresistant mutants of S6K1, another effector of mTOR .
Synonyms: PIA, mAVO3, KIAA1999, MGC39830, DKFZp686B11164, RICTOR

Molecular Weight: 192kDa

Gene ID: 253260

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, CXCR4-mediated Signaling Events