SIAIOSYI-TN antibody

Catalog No. ABIN967028

Product Details

Target: SIAIOSYI-TN

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB)

Specificity: This antibody reacts with sialosyl-Tn antigen, [NeuAca2-6 GalNac-Ser (Thr)]. It shows no cross-reactivity with aGalNac-Ser (Thr).

Purification: Concentrated.

Immunogen: Sialosyl-Tn peptide.

Clone: B35-1

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistochemistry: 1:50-1:100
Western blotting: 1:100-1:500
ELISA 1:2000-1:5000.
Staining Procedure: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. However, prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50-1:100 in IHC. This product can be also used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory.

Comment:

Recommended Positive Control: Colon Carcinoma, Ovarian Mucinous Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References

Itzkowitz, Yuan, Montgomery, Kjeldsen, Takahashi, Bigbee, Kim: "Expression of Tn, sialosyl-Tn, and T antigens in human colon cancer." in: Cancer research, Vol. 49, Issue 1, pp. 197-204, (1989) (PubMed).

Gold, Mattes: "Monoclonal antibody B72.3 reacts with a core region structure of O-linked carbohydrates." in: Tumour biology, Vol. 9, Issue 2-3, pp. 137-44, (1988) (PubMed).

Kjeldsen, Clausen, Hirohashi, Ogawa, Iijima, Hakomori: "Preparation and characterization of monoclonal antibodies directed to the tumor-associated O-linked sialosyl-2----6 alpha-N-acetylgalactosaminyl (sialosyl-Tn) epitope." in: Cancer research, Vol. 48, Issue 8, pp. 2214-20, (1988) (PubMed).

HUMMEL, LITTLE: "Studies on the mouse mammary tumor agent; survival and propagation of the agent in transplanted tumors and in hosts that grew these tumors in their tissues." in: Cancer research, Vol. 9, Issue 3, pp. 137, (1949) (PubMed).

Target Details

Target: SIAIOSYI-TN