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Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pTyr701) antibody

Antigen

Signal Transducer and Activator of Transcription 1, 91kDa (STAT1)

Synonyms ISGF-3, STAT91, DKFZp686B04100, AA408197, 2010005J02Rik, IMUP, H2RSP, IMUP-1, IMUP-2
Binding Site
Alternatives

pTyr701

Host
Alternatives

Rabbit

Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)

Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
2 references available
Catalog no. ABIN967085
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information

Alternative name STAT1
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human STAT1 around the phosphorylation site of tyrosine 701 (T-G-YP-I-K).
Specificity STAT1 (phospho-Tyr701) antibody detects endogenous levels of STAT1 only when phosphorylated at tyrosine 701.

Application Details

Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Research Area Cancer, Transcription Factors, Signaling, Receptors, Inflammation
Restrictions For Research Use only

Publications

Product Heim: "The Jak-STAT pathway: cytokine signalling from the receptor to the nucleus." in: Journal of receptor and signal transduction research, Vol. 19, Issue 1-4, pp. 75-120, 1999 (PubMed).

Demoulin, Van Roost, Stevens et al.: "Distinct roles for STAT1, STAT3, and STAT5 in differentiation gene induction and apoptosis inhibition by interleukin-9." in: The Journal of biological chemistry, Vol. 274, Issue 36, pp. 25855-61, 1999 (PubMed).

Alternatives

Alternatives for antigen "Signal Transducer and Activator of Transcription 1, 91kDa (STAT1)", type "Antibodies"
Hosts Rabbit (142), Mouse (34), Goat (4), Chicken (2), Hamster (2)
Reactivities Human (171), Mouse (Murine) (101), Rat (Rattus) (91), Dog (Canine) (31), Cow (Bovine) (28), Chicken (21), Pig (Porcine) (11), Sheep (Ovine) (5), Monkey (3), Xenopus laevis (2), Zebrafish (1)
Applications Western Blotting (WB) (151), ELISA (44), Immunohistochemistry (IHC) (42), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (41), Immunoprecipitation (IP) (41), Immunofluorescence (IF) (39), Immunocytochemistry (ICC) (9), Flow Cytometry (FACS) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Dot Blot (DB) (1), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1)
Conjugates Alexa Fluor 488 (4), Alexa Fluor 350 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (3), Biotin (2), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pSer727 (23), pTyr701 (20), C-Term (16), N-Term (4), Transcript Variant Beta (4), Ser727 (2), Tyr701 (2), AA 51-235 (1), AA 686-750 (1), AA 711-730 (1), AA 712-750 (1), Internal Region (1), Phosphospecific (1), alpha beta (1), pThr228 (1), pTyr418 (1)