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Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (pSer727) antibody

Antigen

Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3)
Synonyms APRF, HIES, FLJ20882, MGC16063, fc15d02, fl59g06, z-Stat3, MGC193054, wu:fc15d02, wu:fl59g06, STAT3, DKFZp469E1221
Binding Site
Alternatives

pSer727
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
3 references available
Catalog no. ABIN967089
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name STAT3
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human STAT3 around the phosphorylation site of serine 727 (P-M-SP-P-R).
Specificity STAT3 (phospho-Ser727) antibody detects endogenous levels of STAT3 only when phosphorylated at serine 727.

Application Details
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Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Research Area Embryogenesis, Embryonic stem cells, Transcription Factors, Signaling, Receptors, Inflammation
Restrictions For Research Use only

Publications
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Product Takeda, Noguchi, Shi et al.: "Targeted disruption of the mouse Stat3 gene leads to early embryonic lethality." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 8, pp. 3801-4, 1997 (PubMed).

Catlett-Falcone, Landowski, Oshiro et al.: "Constitutive activation of Stat3 signaling confers resistance to apoptosis in human U266 myeloma cells." in: Immunity, Vol. 10, Issue 1, pp. 105-15, 1999 (PubMed).

Heim: "The Jak-STAT pathway: cytokine signalling from the receptor to the nucleus." in: Journal of receptor and signal transduction research, Vol. 19, Issue 1-4, pp. 75-120, 1999 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3)", type "Antibodies"
Hosts Rabbit (174), Mouse (33), Goat (11), Sheep (1)
Reactivities Human (191), Mouse (Murine) (122), Rat (Rattus) (106), Dog (Canine) (40), Cow (Bovine) (35), Pig (Porcine) (31), Chicken (29), Guinea Pig (28), Sheep (Ovine) (28), Monkey (5), Xenopus laevis (2), Chimpanzee (1), Hamster (1), Rhesus Monkey (1), Zebrafish (1), Zebrafish (Danio rerio) (1)
Applications Western Blotting (WB) (175), ELISA (87), Immunohistochemistry (IHC) (71), Immunofluorescence (IF) (53), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (41), Immunoprecipitation (IP) (35), Immunocytochemistry (ICC) (26), Flow Cytometry (FACS) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Gel Shift (GS) (3), Fluorescence Microscopy (FM) (2), Immunoassay (IA) (2), Cellular Assay (CA) (1), Dot Blot (Dot) (1), Electrophoretic Mobility-Shift Assay (EMSA) (1), Enzyme Immunoassay (EIA) (1), Immunoelectron Microscopy (IEM) (1), Immunostaining (ISt) (1)
Conjugates Biotin (6), Alexa Fluor 647 (4), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), FITC (3), PE,Cy5.5 (3), PE (2), CF (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pTyr705 (23), pSer727 (20), C-Term (11), Ser727 (4), AA 50-240 (3), N-Term (3), pTyr705,C-Term (3), Internal Region (2), Isoform 1, Isoform 2 (2), Tyr705 (2), pTyr705,Internal Region (2), AA 1-170 (1), AA 1-175 (1), AA 688-722 (1), Phosphospecific (1), Ser721 (1), Transcript Variant 3 (1), pTyr690 (1), pTyr701 (1), pTyr727 (1), phospho (1)