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Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (pTyr705) antibody

Antigen

Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3)
Synonyms APRF, HIES, FLJ20882, MGC16063, fc15d02, fl59g06, z-Stat3, MGC193054, wu:fc15d02, wu:fl59g06, STAT3, DKFZp469E1221
Binding Site
Alternatives

pTyr705
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
5 references available
Catalog no. ABIN967090
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name STAT3
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human STAT3 around the phosphorylation site of tyrosine 705 (A-P-YP-L-K).
Specificity STAT3 (phospho-Tyr705) antibody detects endogenous levels of STAT3 only when phosphorylated at tyrosine 705.

Application Details
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Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Research Area Embryogenesis, Embryonic stem cells, Transcription Factors, Signaling, Receptors, Inflammation
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Srivastava, Pandey: "Potential mechanism(s) involved in the regulation of glycogen synthesis by insulin." in: Molecular and cellular biochemistry, Vol. 182, Issue 1-2, pp. 135-41, 1999 (PubMed).

Leadbetter: "Cultivation of recalcitrant microbes: cells are alive, well and revealing their secrets in the 21st century laboratory." in: Current opinion in microbiology, Vol. 6, Issue 3, pp. 274-81, 2003 (PubMed).

Barry, Graham, Fry et al.: "Regulation of glycogen synthase kinase 3 in human platelets: a possible role in platelet function?" in: FEBS letters, Vol. 553, Issue 1-2, pp. 173-8, 2003 (PubMed).

Fan, Ballou, Lin: "Phospholipase C-independent activation of glycogen synthase kinase-3beta and C-terminal Src kinase by Galphaq." in: The Journal of biological chemistry, Vol. 278, Issue 52, pp. 52432-6, 2003 (PubMed).

Welsh, Wilson, Proud: "GSK3: a SHAGGY frog story." in: Trends in cell biology, Vol. 6, Issue 7, pp. 274-9, 2004 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3)", type "Antibodies"
Hosts Rabbit (174), Mouse (33), Goat (11), Sheep (1)
Reactivities Human (191), Mouse (Murine) (122), Rat (Rattus) (106), Dog (Canine) (40), Cow (Bovine) (35), Pig (Porcine) (31), Chicken (29), Guinea Pig (28), Sheep (Ovine) (28), Monkey (5), Xenopus laevis (2), Chimpanzee (1), Hamster (1), Rhesus Monkey (1), Zebrafish (1), Zebrafish (Danio rerio) (1)
Applications Western Blotting (WB) (175), ELISA (87), Immunohistochemistry (IHC) (71), Immunofluorescence (IF) (53), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (41), Immunoprecipitation (IP) (35), Immunocytochemistry (ICC) (26), Flow Cytometry (FACS) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Gel Shift (GS) (3), Fluorescence Microscopy (FM) (2), Immunoassay (IA) (2), Cellular Assay (CA) (1), Dot Blot (Dot) (1), Electrophoretic Mobility-Shift Assay (EMSA) (1), Enzyme Immunoassay (EIA) (1), Immunoelectron Microscopy (IEM) (1), Immunostaining (ISt) (1)
Conjugates Biotin (6), Alexa Fluor 647 (4), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), FITC (3), PE,Cy5.5 (3), PE (2), CF (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pTyr705 (22), pSer727 (21), C-Term (11), Ser727 (4), AA 50-240 (3), N-Term (3), pTyr705,C-Term (3), Internal Region (2), Isoform 1, Isoform 2 (2), Tyr705 (2), pTyr705,Internal Region (2), AA 1-170 (1), AA 1-175 (1), AA 688-722 (1), Phosphospecific (1), Ser721 (1), Transcript Variant 3 (1), pTyr690 (1), pTyr701 (1), pTyr727 (1), phospho (1)