Add to Basket
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

CD44 (CD44) antibody

Antigen

CD44 (CD44)
Synonyms IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, CDW44, CSPG8, HCELL, MUTCH-I, ECMR-III, MGC10468
Clonality Monoclonal (IM7)
Host
Reactivity
Alternatives

Mouse (Murine), Human, Cow (Bovine), Monkey, Baboon, Dog (Canine), Cat (Feline), Pig (Porcine)
Application
Alternatives Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
Catalog no. ABIN96712
Quantity 0.5 mg  (0.5 mg/ml)  (Variants)
Price 277.00 $   Plus shipping costs $35.00
Shipping to
Availability Ships within 3 to 10 Business Days

Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Alternative name CD44
Immunogen etics 15:299 2. Lesley, J. et al. (1988) Cell Immunol. 112:40
Isotype IgG2b  (Matching secondary antibodies)
Clone IM7
Specificity Rat Anti-Mouse CD44 (Clone IM7) recognizes recognizes all isoforms of the Pgp-1 cell surface glycoprotein. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Antigen Distribution: The CD44 antigen is present on B-cells, monocytes, macrophages and certain subsets of thymocytes and peripheral T-cells. Mice with the Ly-24.1 allotype have high densities of CD44+ T-cells, while Ly-24.2 strains are low expressors. The antibody cross reacts with Human on canine.

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes IHC (Frozen), FC, IP Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD44. A suggested method would be to stain cells expressing CD44 with 0.125 g per 1 x 106 cells in a total staining volume of 100 l followed by Goat Anti-Rat IgG (H&L)-FITC (Leinco Prod. No.: R1208). Each Investigator should determine their own optimal working dilution for specific applications.
Concentration 0.5 mg/ml
Purity >98% by SDS Page and HPLC
Buffer Low Endotoxin Functional Formulation
Storage This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at 80°C. Avoid Repeated Freeze Thaw Cycles.
Research Area CD Antigens
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "CD44 (CD44)", type "Antibodies"
Hosts Mouse (295), Rabbit (130), Rat (105), Goat (2), Human (1)
Reactivities Human (346), Mouse (Murine) (194), Rat (Rattus) (115), Dog (Canine) (62), Pig (Porcine) (51), Rabbit (51), Cow (Bovine) (34), Chicken (33), Horse (Equine) (28), Cat (Feline) (12), Monkey (12), Sheep (Ovine) (8), Baboon (4), Bird (Avian) (4), Goat (2)
Applications Flow Cytometry (FACS) (411), Western Blotting (WB) (132), Immunohistochemistry (Frozen Sections) (IHC (fro)) (119), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (119), Immunoprecipitation (IP) (108), Immunofluorescence (IF) (107), ELISA (52), Immunohistochemistry (IHC) (28), Enzyme Immunoassay (EIA) (11), Immunocytochemistry (ICC) (8), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (8), Functional Studies (Func) (7), Cytotoxicity Test (CyTox) (6), Blocking Antibody (Inhibition) (5), Immunoelectron Microscopy (IEM) (5), ELISA (Detection) (2), Radioimmunoassay (RIA) (2), Blocking Antibody (Inhibition) (1), Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) (1), Neutralization (Neut) (1)
Conjugates FITC (92), Biotin (48), PE (38), APC (15), RPE (9), Cy5.5 (7), PE,Cy7 (7), Alexa Fluor 488 (6), Alexa Flour 488 (5), Alexa Fluor 350 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), Cy3 (5), Cy5 (5), Cy7 (5), Gold (5), HRP (5), PE,Cy3 (5), PE,Cy5 (5), PE,Cy5.5 (5), RBITC (5), Cy5,PE (3), Cy5.5,PE (3), SPRD (3), Cy7,PE (2), Alexa Fluor 700 (1), Pacific Blue (1)
Epitopes pSer706 (1)