Microtubule-Associated Protein tau (MAPT) (pSer404) antibody

Antigen: Microtubule-Associated Protein tau (MAPT)

Synonyms: TAU, MSTD, PPND, DDPAC, MAPTL, MTBT1, MTBT2, FTDP-17, FLJ31424, MGC138549, Tau, Mtapt, AI413597, AW045860, pTau, RNPTAU, MGC156663, tau, MGC134287, xtp, MAPT

Binding Site: pSer404

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN967147

Additional Information

Alternative name: Tau

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Tau around the phosphorylation site of serine 404 (D-T-SP-P-R).

Specificity: Tau (phospho-Ser404) antibody detects endogenous levels of Tau only when phosphorylated at serine404.

Application Details

Application Notes: WB: 1:500~1:1000.

Concentration: 100ug/100ul.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: 0.02% Sodium Azide

Storage: Store at -20°C/1 year.

Research Area: Neurology, Cytoskeleton, Alzheimer's Disease

Restrictions: For Research Use only

Publications

Product: Andreadis, Brown, Kosik: "Structure and novel exons of the human tau gene." in: Biochemistry, Vol. 31, Issue 43, pp. 10626-33, 1992 (PubMed).

Lee, Neve, Kosik: "The microtubule binding domain of tau protein." in: Neuron, Vol. 2, Issue 6, pp. 1615-24, 1990 (PubMed).

Giasson, Sampathu, Wilson et al.: "The environmental toxin arsenite induces tau hyperphosphorylation." in: Biochemistry, Vol. 41, Issue 51, pp. 15376-87, 2002 (PubMed).

Noble, Olm, Takata et al.: "Cdk5 is a key factor in tau aggregation and tangle formation in vivo." in: Neuron, Vol. 38, Issue 4, pp. 555-65, 2003 (PubMed).

Gustin, Ozes, Akca et al.: "Cell type-specific expression of the IkappaB kinases determines the significance of phosphatidylinositol 3-kinase/Akt signaling to NF-kappa B activation." in: The Journal of biological chemistry, Vol. 279, Issue 3, pp. 1615-20, 2004 (PubMed).

Li, Yin, Kuret: "Casein kinase 1 delta phosphorylates tau and disrupts its binding to microtubules." in: The Journal of biological chemistry, Vol. 279, Issue 16, pp. 15938-45, 2004 (PubMed).