UBA7 antibody

Catalog No. ABIN967228

Product Details anti-UBA7 Antibody

Target: UBA7 (Ubiquitin-Like Modifier Activating Enzyme 7 (UBA7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This UBA7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human UBE1L expressed in E. Coli.

Clone: 5B10

Isotype: IgG1

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-UBA7 antibody (ABIN967228)

Kitareewan, Pitha-Rowe, Sekula, Lowrey, Nemeth, Golub, Freemantle, Dmitrovsky: "UBE1L is a retinoid target that triggers PML/RARalpha degradation and apoptosis in acute promyelocytic leukemia." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 6, pp. 3806-11, (2002) (PubMed).

Carritt, Kok, van den Berg, Osinga, Pilz, Hofstra, Davis, van der Veen, Rabbitts, Gulati: "A gene from human chromosome region 3p21 with reduced expression in small cell lung cancer." in: Cancer research, Vol. 52, Issue 6, pp. 1536-41, (1992) (PubMed).

Target Details for UBA7

Target: UBA7 (Ubiquitin-Like Modifier Activating Enzyme 7 (UBA7))

Alternative Name: UBE1L (UBA7 Products)

Synonyms: D8 antibody, UBA1B antibody, UBE1L antibody, UBE2 antibody, Ube1l antibody, 1300004C08Rik antibody, UBE1 antibody, xubc3 antibody, ubiquitin like modifier activating enzyme 7 antibody, ubiquitin-like modifier activating enzyme 7 antibody, ubiquitin conjugating enzyme E2 R2 S homeolog antibody, ubiquitin-like modifier-activating enzyme 7 antibody, UBA7 antibody, Uba7 antibody, ube2r2.S antibody, LOC100092397 antibody

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting.
Tissue specificity: Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferonstimulated gene 15 protein.
Synonyms: D8, UBE2, UBA1B, UBE1L, MGC12713, UBA7

Molecular Weight: 112kDa

Gene ID: 7318