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Vimentin antibody (VIM)

Details for Product anti-VIM Antibody No. ABIN967251, Supplier: Log in to see
Antigen
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Alternatives
anti-Human Vimentin antibody for Immunohistochemistry (Frozen Sections)
Reactivity
Human
857
371
317
221
139
132
115
84
79
50
49
42
41
36
30
10
7
7
5
5
4
3
3
2
2
2
1
1
1
1
1
1
Host
530
302
30
15
12
8
7
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
28
28
23
20
15
14
12
11
11
11
11
11
11
11
11
9
5
5
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
ELISA
684
301
283
267
248
221
217
108
84
40
15
14
13
10
8
8
5
3
3
3
3
1
1
1
1
1
Options
Supplier
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Isotype IgG1
Specificity Ni-NTA purified truncated recombinant Vimentin expressed in E. Coli strain BL21 (DE3)
Purification Antibodies are purified by protein A affinity chromatography
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Gene ID 7431
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis
Application Notes ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Storage -20 °C
Product cited in: Vanzi: "Freedom at home: state constitutions and Medicaid funding for abortions." in: New Mexico law review, Vol. 26, Issue 3, pp. 433-54, 2005 (PubMed).

Seshadri, Raymond, Leong, Horsfall, McCaul: "Vimentin expression is not associated with poor prognosis in breast cancer." in: International journal of cancer. Journal international du cancer, Vol. 67, Issue 3, pp. 353-6, 1996 (PubMed).

Chu, Seftor, Romer, Hendrix: "Experimental coexpression of vimentin and keratin intermediate filaments in human melanoma cells augments motility." in: The American journal of pathology, Vol. 148, Issue 1, pp. 63-9, 1996 (PubMed).