XRCC4 antibody (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 4) (C-Term)

Details for Product anti-XRCC4 Antibody No. ABIN967265, Supplier: Log in to see
Antigen
  • zgc:73312
  • MGC81443
  • homolog of human DNA ligase iv-binding protein XRCC4
  • 2310057B22Rik
  • AW413319
  • AW545101
  • X-ray repair complementing defective repair in Chinese hamster cells 4
  • DNA repair protein XRCC4-like protein
  • XRCC4
  • xrcc4
  • Xrcc4
Alternatives
anti-Human XRCC4 antibody for ELISA
Epitope
C-Term, sumoLys210
15
12
8
8
7
6
5
4
4
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
108
27
24
2
2
2
2
1
1
1
1
1
1
1
Host
Rabbit
96
12
Clonality
Polyclonal
Conjugate
This XRCC4 antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC)
88
26
23
16
14
13
9
7
1
1
1
1
Options
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Immunogen Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to C-residues of human XRCC4(X-ray repair cross-complementing protein 4)
Note: Antigen containing K210 amino group with the SUMO1 c-terminal 7-mer peptide bound: YQEQTGG
Alternative Name XRCC4 (XRCC4 Antibody Abstract)
Background XRCC4 (X-ray repair cross-complementing protein 4) is involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. XRCC4 binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. XRCC4 is a homodimer and homotetramer in solution. The homodimer associates with LIG4, and the LIG4-XRCC4 complex associates in a DNA-dependent manner with the DNA-PK complex formed by the Ku p70/p86 dimer (G22P1/G22P2) and PRKDC. XRCC4 seems to interact directly with PRKDC but not with the Ku p70/86 dimer. It interacts with XLF/Cernunnos. Interacts with APTX and APLF. Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. It has no effect on ubiquitination.
Pathways DNA Damage Repair, Production of Molecular Mediator of Immune Response
Restrictions For Research Use only
Background publications Chen, Trujillo, Sung, Tomkinson: "Interactions of the DNA ligase IV-XRCC4 complex with DNA ends and the DNA-dependent protein kinase." in: The Journal of biological chemistry, Vol. 275, Issue 34, pp. 26196-205, 2000 (PubMed).

Li, Otevrel, Gao, Cheng, Seed, Stamato, Taccioli, Alt: "The XRCC4 gene encodes a novel protein involved in DNA double-strand break repair and V(D)J recombination." in: Cell, Vol. 83, Issue 7, pp. 1079-89, 1996 (PubMed).

Product cited in: Nick McElhinny, Snowden, McCarville, Ramsden: "Ku recruits the XRCC4-ligase IV complex to DNA ends." in: Molecular and cellular biology, Vol. 20, Issue 9, pp. 2996-3003, 2000 (PubMed).

Leber, Wise, Mizuta, Meek: "The XRCC4 gene product is a target for and interacts with the DNA-dependent protein kinase." in: The Journal of biological chemistry, Vol. 273, Issue 3, pp. 1794-801, 1998 (PubMed).

Grawunder, Wilm, Wu, Kulesza, Wilson, Mann, Lieber: "Activity of DNA ligase IV stimulated by complex formation with XRCC4 protein in mammalian cells." in: Nature, Vol. 388, Issue 6641, pp. 492-5, 1997 (PubMed).