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XRCC4 antibody (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 4) (C-Term)

Details for Product anti-XRCC4 Antibody No. ABIN967265, Supplier: Log in to see
Antigen
  • zgc:73312
  • MGC81443
  • homolog of human DNA ligase iv-binding protein XRCC4
  • 2310057B22Rik
  • AW413319
  • AW545101
Alternatives
anti-Human XRCC4 antibody for Western Blotting
Epitope
C-Term, sumoLys210
16
15
13
7
5
5
4
3
3
2
2
1
1
1
1
1
1
1
Reactivity
Human
99
21
20
1
Host
Rabbit
87
12
Clonality
Polyclonal
Conjugate
This XRCC4 antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC)
81
24
21
12
11
11
9
3
1
1
1
1
Supplier
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Immunogen Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to C-residues of human XRCC4(X-ray repair cross-complementing protein 4)
Note: Antigen containing K210 amino group with the SUMO1 c-terminal 7-mer peptide bound: YQEQTGG
Alternative Name XRCC4 (XRCC4 Antibody Abstract)
Background XRCC4 (X-ray repair cross-complementing protein 4) is involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. XRCC4 binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. XRCC4 is a homodimer and homotetramer in solution. The homodimer associates with LIG4, and the LIG4-XRCC4 complex associates in a DNA-dependent manner with the DNA-PK complex formed by the Ku p70/p86 dimer (G22P1/G22P2) and PRKDC. XRCC4 seems to interact directly with PRKDC but not with the Ku p70/86 dimer. It interacts with XLF/Cernunnos. Interacts with APTX and APLF. Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. It has no effect on ubiquitination.
Pathways DNA Damage Repair
Restrictions For Research Use only
Product cited in: Nick McElhinny, Snowden, McCarville et al.: "Ku recruits the XRCC4-ligase IV complex to DNA ends." in: Molecular and cellular biology, Vol. 20, Issue 9, pp. 2996-3003, 2000 (PubMed).

Leber, Wise, Mizuta et al.: "The XRCC4 gene product is a target for and interacts with the DNA-dependent protein kinase." in: The Journal of biological chemistry, Vol. 273, Issue 3, pp. 1794-801, 1998 (PubMed).

Grawunder, Wilm, Wu et al.: "Activity of DNA ligase IV stimulated by complex formation with XRCC4 protein in mammalian cells." in: Nature, Vol. 388, Issue 6641, pp. 492-5, 1997 (PubMed).

Background publications Chen, Trujillo, Sung et al.: "Interactions of the DNA ligase IV-XRCC4 complex with DNA ends and the DNA-dependent protein kinase." in: The Journal of biological chemistry, Vol. 275, Issue 34, pp. 26196-205, 2000 (PubMed).

Li, Otevrel, Gao et al.: "The XRCC4 gene encodes a novel protein involved in DNA double-strand break repair and V(D)J recombination." in: Cell, Vol. 83, Issue 7, pp. 1079-89, 1996 (PubMed).