You are viewing an incomplete version of our website. Please click to reload the website as full version.

ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (pTyr493)

Details for Product anti-ZAP70 Antibody No. ABIN967270, Supplier: Log in to see
Antigen
  • 70kDa
  • AI327364
  • MGC80670
  • MGC88962
  • mrtle
  • mur
  • SRK
  • Srk
  • STCD
  • STD
  • TZK
  • ZAP-70
  • zap70
  • ZAP70
Alternatives
anti-Human ZAP70 antibody for Western Blotting
Epitope
pTyr493
49
47
46
25
22
21
21
20
15
15
15
14
12
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
456
170
118
4
4
3
2
2
2
Host
Rabbit
295
141
15
8
3
Conjugate
This ZAP70 antibody is un-conjugated
29
21
16
14
10
7
5
5
4
4
4
4
4
4
4
1
Application
Immunohistochemistry (IHC)
279
162
150
82
62
44
39
38
10
7
5
4
4
3
2
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Zap-70 around the phosphorylation site of tyrosine 493 (S-Y-YP-T-A).
Specificity Zap-70 (phospho-Tyr493) antibody detects endogenous levels of Zap-70 only when phosphorylated at tyrosine 493
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name Zap-70 (ZAP70 Antibody Abstract)
Research Area Signaling, Tags/Labels, Immunology, Cancer
Pathways TCR Signaling
Application Notes IHC: 1:50-1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Salomon, Ficarro, Brill, Brinker, Phung, Ericson, Sauer, Brock, Horn, Schultz, Peters: "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 443-8, 2003

Background publications Kong, Dalton, Bubeck Wardenburg, Straus, Kurosaki, Chan: "Distinct tyrosine phosphorylation sites in ZAP-70 mediate activation and negative regulation of antigen receptor function." in: Molecular and cellular biology, Vol. 16, Issue 9, pp. 5026-35, 1996

Chan, Dalton, Johnson, Kong, Wang, Thoma, Kurosaki: "Activation of ZAP-70 kinase activity by phosphorylation of tyrosine 493 is required for lymphocyte antigen receptor function." in: The EMBO journal, Vol. 14, Issue 11, pp. 2499-508, 1995