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zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) antibody

Antigen

zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)
Synonyms SRK, STD, TZK, ZAP-70, FLJ17670, FLJ17679, Srk, mur, 70kDa, mrtle, AI327364, MGC108902, zap70, MGC80670, MGC88962, ZAP70
Binding Site
Alternatives

pTyr493
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
3 references available
Catalog no. ABIN967270
Quantity 100ug  (100ug/100ul.)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name Zap-70
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Zap-70 around the phosphorylation site of tyrosine 493 (S-Y-YP-T-A).
Specificity Zap-70 (phospho-Tyr493) antibody detects endogenous levels of Zap-70 only when phosphorylated at tyrosine 493

Application Details
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Application Notes IHC: 1:50-1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Research Area Signaling, Tags/Labels, Immunology, Cancer
Restrictions For Research Use only

Publications
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Product Chan, Dalton, Johnson et al.: "Activation of ZAP-70 kinase activity by phosphorylation of tyrosine 493 is required for lymphocyte antigen receptor function." in: The EMBO journal, Vol. 14, Issue 11, pp. 2499-508, 1995 (PubMed).

Kong, Dalton, Bubeck Wardenburg et al.: "Distinct tyrosine phosphorylation sites in ZAP-70 mediate activation and negative regulation of antigen receptor function." in: Molecular and cellular biology, Vol. 16, Issue 9, pp. 5026-35, 1996 (PubMed).

Salomon, Ficarro, Brill et al.: "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 443-8, 2003 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)", type "Antibodies"
Hosts Rabbit (123), Mouse (75), Goat (4), Sheep (1)
Reactivities Human (201), Mouse (Murine) (90), Rat (Rattus) (68), Cow (Bovine) (32), Pig (Porcine) (29), Horse (Equine) (28), Dog (Canine) (26), Chicken (24), Guinea Pig (23), Cat (Feline) (1), Rabbit (1)
Applications Western Blotting (WB) (109), ELISA (70), Flow Cytometry (FACS) (41), Immunohistochemistry (IHC) (39), Immunofluorescence (IF) (38), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (33), Immunoprecipitation (IP) (24), Immunocytochemistry (ICC) (23), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), Dot Blot (Dot) (4), Immunohistochemistry (Fixed) (IHC (fx)) (2), Immunoelectron Microscopy (IEM) (1)
Conjugates FITC (12), PE (9), Biotin (7), HRP (4), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), Alkaline Phosphatase (AP) (3), PE,Cy5.5 (3), RPE (2), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pTyr493 (13), AA 280-309 (10), pTyr319 (10), C-Term (9), AA 282-307 (6), Internal Region (6), pTyr292 (5), pTyr319,pTyr315 (5), AA 1-254 (4), AA 326-341 (3), N-Term (3), Center (2), Tyr319 (2), Tyr493 (2), pTyr492 (2), AA 10-102 (1), AA 163-254 (1), Middle Region (1), pSer102 (1), pTyr315 (1), pTyr315, pTyr319 (1)