Cytokeratin 5/8 antibody

Antigen: Cytokeratin 5/8

Clonality: Monoclonal (RCK102)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN967288

Additional Information

Immunogen: Human lung cancer cell line (MR21)

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Hamster, Rabbit, Dog (Canine), Pig (Porcine)

Format: Liquid

Isotype: IgG1

Clone: RCK102

Description: Intermediate filaments (IF) are a subset of cytoskeletal proteins which function to give overall structural integrity to the plasma membrane as well as organize cells into specific tissues. IF proteins can be divided into six major types based upon the similarity in sequence. Type I and II IF proteins are designated keratins based upon whether they are acidic (I) or basic (II) and are characterized by their remarkable biochemical diversity. A subgroup of keratins, designated cytokeratins, are represented in epithelial tissues by at least 20 different polypeptides, designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin 5 has a molecular weight of approximately 58 kDa (NCBI Accession number P13647) while cytokeratin 8 is approximately 53 kDa (NCBI Accession number P05787). We observe cytokeratin 5 to migrate at approximately 54 kDa and cytokeratin 8 at approximately 52 kDa. The antibody recognizes cytokeratins 5 and 8 from human, mouse, rat, hamster, rabbit, canine, and swine. The antibody stains all epithelial tissue, it does not stain non-epithelial tissue. The human lung cancer cell line (MR21) was used as the immunogen.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 54/52 kDa

Comments:

Related Products: ABIN968535, ABIN967389

Application Details

Application Notes: Applications include western blot analysis (0.5 - 1 µg/ml). HeLa cell lysate (ABIN968535) is recommended as a positive control. Additional reported applications include immunohistochemistry on methanol fixed tissue, but this is application is not tested.

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Research Area: Extracellular Matrix

Restrictions: For Research Use only

Images

Western blot analysis of Cytokeratin 5/8. Lysate from HeLa cells was probed with anti-cytokeratin 5/8 (clone RCK102, ABIN967288) at concentrations of 1 (lane 1), 0.5 (lane 2), and 0.25 µg/ml (lane 3). Cytokeratin 5/8 is identified as a doublet of ~54/52 kDa.

Publications

Product: Smedts, Ramaekers, Robben et al.: "Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia." in: The American journal of pathology, Vol. 136, Issue 3, pp. 657-68, 1990 (PubMed).

Broers, Carney, Klein Rot et al.: "Intermediate filament proteins in classic and variant types of small cell lung carcinoma cell lines: a biochemical and immunochemical analysis using a panel of monoclonal and polyclonal antibodies." in: Journal of cell science, Vol. 83, Issue 12, pp. 37-60, 1987 (PubMed).

Ramaekers, Huysmans, Schaart et al.: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, 1987 (PubMed).

Broers, Ramaekers, Rot et al.: "Cytokeratins in different types of human lung cancer as monitored by chain-specific monoclonal antibodies." in: Cancer research, Vol. 48, Issue 11, pp. 3221-9, 1988 (PubMed).