BCL2-Like 2 (BCL2L2) (full length) antibody

Antigen: BCL2-Like 2 (BCL2L2)

Synonyms: bcl2l2, BCL2L2, BCLW, BCL-W, KIAA0271

Binding Site: full length

Clonality: Monoclonal (16H12)

Host: Rat

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN967292

Additional Information

Alternative name: Bcl-w

Immunogen: Human Bcl-w full-length Recombinant Protein

Cross-Reactivity: Mouse (Murine)

Format: Liquid

Isotype: IgG2a

Clone: 16H12

Description: The Bcl-2 family of proteins plays a pivotal role in determining the life and death of a cell. Members of the Bcl-2 family all possess at least one of four conserved Bcl-2 homology domains (BH1-BH4) which mediate protein-protein interactions. They can be divided into molecules that have either an anti-apoptotic or pro-apoptotic function. The ratio between these two groups of molecules can help determine whether a cell lives or dies. The majority of anti-apoptotic family members contain common BH1-BH4 domains and are most similar to Bcl-2. Bcl-w is a member of the antiapoptotic Bcl-2 group of proteins, including Bcl-x, Mcl-1, A1/Bfl-1, Boo/Diva, and Nr-13. Bcl-w was cloned from a mouse macrophage cell line and mouse brain. Bcl-w was found to be expressed in cells of myeloid origin, as well as many other tissues. The Bcl-w gene was inactivated in mice by homologous recombination and the resulting mice were viable and normal in appearance. Tissue histology demonstrated that male mice had reduced Sertoli and germ cells as well as having defective seminiferous tubules, resulting in infertility, while female mice had normal reproductive function.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.

Molecular Weight: 21-25 kDa

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide, 0.09% Bronidox.

Storage: Store undiluted at 4°C.

Restrictions: For Research Use only

Images

Western blot analysis for Bcl-w. Lysates from mouse brain tissue were probed with the rat anti- Bcl-w antibody at concentrations of 0.5 µg/mL (lane 1), 0.25 µg/mL (lane 2), and 0.125 µg/mL (lane 3). Bcl-w may be observed to be migrating in a range from 21-25 kDa.

Publications

Product: Oltvai, Milliman, Korsmeyer: "Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death." in: Cell, Vol. 74, Issue 4, pp. 609-19, 1993 (PubMed).

Gibson, Holmgreen, Huang et al.: "bcl-w, a novel member of the bcl-2 family, promotes cell survival." in: Oncogene, Vol. 13, Issue 4, pp. 665-75, 1996 (PubMed).

Adams, Cory: "The Bcl-2 protein family: arbiters of cell survival." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1322-6, 1998 (PubMed).

Print, Loveland, Gibson et al.: "Apoptosis regulator bcl-w is essential for spermatogenesis but appears otherwise redundant." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 21, pp. 12424-31, 1998 (PubMed).

Gross, McDonnell, Korsmeyer: "BCL-2 family members and the mitochondria in apoptosis." in: Genes & development, Vol. 13, Issue 15, pp. 1899-911, 1999 (PubMed).