BCL2-Like 2 (BCL2L2) (full length) antibody
|Synonyms||bcl2l2, BCL2L2, BCLW, BCL-W, KIAA0271|
Alternatives Western Blotting (WB)
|5 references available|
|Quantity||50 µg (0.5 mg/ml)|
|Price||Product not available in this region.|
|Immunogen||Human Bcl-w full-length Recombinant Protein|
|Description||The Bcl-2 family of proteins plays a pivotal role in determining the life and death of a cell. Members of the Bcl-2 family all possess at least one of four conserved Bcl-2 homology domains (BH1-BH4) which mediate protein-protein interactions. They can be divided into molecules that have either an anti-apoptotic or pro-apoptotic function. The ratio between these two groups of molecules can help determine whether a cell lives or dies. The majority of anti-apoptotic family members contain common BH1-BH4 domains and are most similar to Bcl-2. Bcl-w is a member of the antiapoptotic Bcl-2 group of proteins, including Bcl-x, Mcl-1, A1/Bfl-1, Boo/Diva, and Nr-13. Bcl-w was cloned from a mouse macrophage cell line and mouse brain. Bcl-w was found to be expressed in cells of myeloid origin, as well as many other tissues. The Bcl-w gene was inactivated in mice by homologous recombination and the resulting mice were viable and normal in appearance. Tissue histology demonstrated that male mice had reduced Sertoli and germ cells as well as having defective seminiferous tubules, resulting in infertility, while female mice had normal reproductive function.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
|Molecular Weight||21-25 kDa|
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution.|
|Preservative||0.09% Sodium azide, 0.09% Bronidox.|
|Storage||Store undiluted at 4°C.|
|Restrictions||For Research Use only|
Oltvai, Milliman, Korsmeyer: "Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death." in: Cell, Vol. 74, Issue 4, pp. 609-19, 1993 (PubMed).
Gibson, Holmgreen, Huang et al.: "bcl-w, a novel member of the bcl-2 family, promotes cell survival." in: Oncogene, Vol. 13, Issue 4, pp. 665-75, 1996 (PubMed).
Adams, Cory: "The Bcl-2 protein family: arbiters of cell survival." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1322-6, 1998 (PubMed).
Print, Loveland, Gibson et al.: "Apoptosis regulator bcl-w is essential for spermatogenesis but appears otherwise redundant." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 21, pp. 12424-31, 1998 (PubMed).
Gross, McDonnell, Korsmeyer: "BCL-2 family members and the mitochondria in apoptosis." in: Genes & development, Vol. 13, Issue 15, pp. 1899-911, 1999 (PubMed).
|Hosts||Rabbit (33), Rat (2), Goat (1), Mouse (1)|
|Reactivities||Human (37), Rat (Rattus) (24), Mouse (Murine) (23), Dog (Canine) (19), Horse (Equine) (18), Pig (Porcine) (18), Cow (Bovine) (2), Monkey (2)|
|Applications||Western Blotting (WB) (20), Immunofluorescence (IF) (19), Flow Cytometry (FACS) (15), ELISA (12), Immunohistochemistry (IHC) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunoprecipitation (IP) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Cellular Assay (CA) (2), Immunoassay (IA) (2), Immunostaining (ISt) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||BH3 Domain (3), AA 16-29 (2), C-Term (1), N-Term (1)|