DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) (AA 203-218) antibody

Antigen: DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB)

Synonyms: CAD, CPAN, DFF2, DFF40, DFF-40, DFFB, LOC100223514

Binding Site: AA 203-218

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN967293

Additional Information

Alternative name: DFF40/CAD

Immunogen: Human DFF40 peptide

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Format: Liquid

Isotype: Ig

Description: The DNA fragmentation factor (DFF) is a complex composed of two subunits, DFF40/CAD and DFF45. DFF40/CAD is the nuclease responsible for DNA fragmentation during apoptosis. DFF45 is a molecular chaperone that inhibits the activity of DFF40/CAD. During apoptosis, activated caspase-3 cleaves DFF45 (45 kDa) into 30 and 11 kDa fragments, resulting in the release and activation of DFF40. Activated DFF40 causes DNA fragmentation which has long been considered to be a hallmark of apoptotic cell death. DFF40 migrates at approximately 40 kDa in SDS/PAGE. The antibodies recognize human, mouse, and rat DFF40/CAD. A peptide corresponding to amino acids (a.a.) 203 to 218 of human DFF40 was used as the immunogen.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.

Molecular Weight: 40 kDa

Comments:

Related Products: ABIN967390, ABIN968537

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from antiserum by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Research Area: Apoptosis/Necrosis, DNA/RNA

Restrictions: For Research Use only

Images

Western blot analysis of DFF40/CAD. A Jurkat cell lysate (Human T-cell leukemia, ATCC TIB-152) was probed with the polyclonal Rabbit Anti-DFF40/CAD antibody at 1.0 µg/mL (lane 1), 0.5 µg/mL (lane 2), and 0.25 µg/ml (lane 3). DFF40 is identified as a band of ~40 kDa.

Publications

Product: Enari, Sakahira, Yokoyama et al.: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998 (PubMed).

Liu, Li, Widlak et al.: "The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 15, pp. 8461-6, 1998 (PubMed).

Mukae, Enari, Sakahira et al.: "Molecular cloning and characterization of human caspase-activated DNase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 16, pp. 9123-8, 1998 (PubMed).

Liu, Zou, Widlak et al.: "Activation of the apoptotic endonuclease DFF40 (caspase-activated DNase or nuclease). Oligomerization and direct interaction with histone H1." in: The Journal of biological chemistry, Vol. 274, Issue 20, pp. 13836-40, 1999 (PubMed).