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DFFB antibody (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase)) (AA 203-218)

Details for Product anti-DFFB Antibody No. ABIN967293, Supplier: Login to see
Antigen
  • LOC100223514
  • CAD
  • CPAN
  • DFF-40
  • DFF2
  • DFF40
  • Cad
  • 40kDa
  • 5730477D02Rik
  • Didff
Alternatives
anti-Human DFFB antibody for Western Blotting
Epitope
AA 203-218
27
18
13
10
8
6
5
5
4
3
3
3
3
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
102
72
48
1
Host
Rabbit
130
10
Clonality
Polyclonal
Conjugate
This DFFB antibody is un-conjugated
5
5
4
2
2
2
2
2
2
2
2
2
2
2
2
Application
Western Blotting (WB)
111
38
25
24
22
20
17
6
5
3
3
1
Supplier
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Brand BD Pharmingen™
Immunogen Human DFF40 peptide aa. 203-218
Isotype Ig Fraction
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
Purification The polyclonal antibody was purified from antiserum by affinity chromatography.
Alternative Name DFF40/CAD (DFFB Antibody Abstract)
Background The DNA fragmentation factor (DFF) is a complex composed of two subunits, DFF40/CAD and DFF45. DFF40/CAD is the nuclease responsible for DNA fragmentation during apoptosis. DFF45 is a molecular chaperone that inhibits the activity of DFF40/CAD. During apoptosis, activated caspase-3 cleaves DFF45 (45 kDa) into 30 and 11 kDa fragments, resulting in the release and activation of DFF40. Activated DFF40 causes DNA fragmentation which has long been considered to be a hallmark of apoptotic cell death. DFF40 migrates at approximately 40 kDa in SDS/PAGE. The antibodies recognize human, mouse, and rat DFF40/CAD. A peptide corresponding to amino acids (a.a.) 203 to 218 of human DFF40 was used as the immunogen.
Molecular Weight 40 kDa
Research Area Apoptosis/Necrosis, DNA/RNA
Pathways Apoptosis, Caspase Cascade in Apoptosis
Comment

Related Products: ABIN967390, ABIN968537

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C. The polyclonal antibody was purified from antiserum by affinity chromatography.
Supplier Images
Western Blotting (WB) image for anti-DFFB antibody (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase)) (AA 203-218) (ABIN967293) Western blot analysis of DFF40/CAD. A Jurkat cell lysate (Human T-cell leukemia, ATCC...
Product cited in: Liu, Zou, Widlak et al.: "Activation of the apoptotic endonuclease DFF40 (caspase-activated DNase or nuclease). Oligomerization and direct interaction with histone H1." in: The Journal of biological chemistry, Vol. 274, Issue 20, pp. 13836-40, 1999 (PubMed).

Mukae, Enari, Sakahira et al.: "Molecular cloning and characterization of human caspase-activated DNase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 16, pp. 9123-8, 1998 (PubMed).

Liu, Li, Widlak et al.: "The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 15, pp. 8461-6, 1998 (PubMed).

Enari, Sakahira, Yokoyama et al.: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998 (PubMed).