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DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) (AA 203-218) antibody

Details for Product No. ABIN967293
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Antigen
Synonyms CAD, CPAN, DFF2, DFF40, DFF-40, 40kDa, Didff, 5730477D02Rik, Cad, LOC100223514
Epitope
AA 203-218
(16), (10), (9), (8), (7), (4), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(83), (69), (46), (12), (11), (2)
Host
Rabbit
(112), (9)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Western Blotting (WB)
(100), (44), (30), (21), (19), (14), (10), (8), (2), (2), (1), (1), (1)
Pubmed 4 references available
Quantity 50 µg
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Catalog No. ABIN967293
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Immunogen Human DFF40 peptide
Isotype Ig
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
Purification Purified from antiserum by affinity chromatography.
Purity Purified
Alternative Name DFF40/CAD
Background The DNA fragmentation factor (DFF) is a complex composed of two subunits, DFF40/CAD and DFF45. DFF40/CAD is the nuclease responsible for DNA fragmentation during apoptosis. DFF45 is a molecular chaperone that inhibits the activity of DFF40/CAD. During apoptosis, activated caspase-3 cleaves DFF45 (45 kDa) into 30 and 11 kDa fragments, resulting in the release and activation of DFF40. Activated DFF40 causes DNA fragmentation which has long been considered to be a hallmark of apoptotic cell death. DFF40 migrates at approximately 40 kDa in SDS/PAGE. The antibodies recognize human, mouse, and rat DFF40/CAD. A peptide corresponding to amino acids (a.a.) 203 to 218 of human DFF40 was used as the immunogen.
Molecular Weight 40 kDa
Research Area Apoptosis/Necrosis, DNA/RNA
Comment

Related Products: ABIN967390, ABIN968537

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) (AA 203-218) antibody Western blot analysis of DFF40/CAD. A Jurkat cell lysate (Human T-cell leukemia, ATCC TIB-152) was probed with the polyclonal Rabbit Anti-DFF40/CAD antibody at 1.0 µg/mL (lane 1), 0.5 µg/mL (lane 2), and 0.25 µg/ml (lane 3). DFF40 is identified as a band of ~40 kDa.
Product cited in: Enari, Sakahira, Yokoyama et al.: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998 (PubMed).

Liu, Li, Widlak et al.: "The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 15, pp. 8461-6, 1998 (PubMed).

Mukae, Enari, Sakahira et al.: "Molecular cloning and characterization of human caspase-activated DNase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 16, pp. 9123-8, 1998 (PubMed).

Liu, Zou, Widlak et al.: "Activation of the apoptotic endonuclease DFF40 (caspase-activated DNase or nuclease). Oligomerization and direct interaction with histone H1." in: The Journal of biological chemistry, Vol. 274, Issue 20, pp. 13836-40, 1999 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN967293
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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