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Details for Product No. ABIN967295

DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 312-331) antibody

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Antigen
Synonyms DFF1, ICAD, DFF-45, DFF35, Dff45, ICAD-L, ICAD-S, A330085O09Rik, DFFA
Epitope
»Alternatives AA 312-331
Reactivity
»Alternatives Mouse (Murine)
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB)
Pubmed 4 references available
Catalog no. ABIN967295
Quantity 200 µL
Price
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Immunogen Mouse ICAD peptide
Isotype IgG
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from antiserum by negative adsorption and affinity chromatography.
Purity Purified
Alternative Name ICAD
Background Apoptosis is essential in maintaining normal cellular homeostasis and for animal development. Morphological as well as biochemical changes are a hallmark of apoptosis and include membrane blebbing, chromosomal condensation and DNA fragmentation. These changes are caused by a class of cysteine proteases called caspases, which, upon activation can cleave multiple cytoplasmic and nuclear substrates. Caspases, once activated by a proapoptotic signal can induce a DNase, designated DFF/CAD to cleave these nuclear substrates. In growing, non-apoptotic cells DFF/CAD is complexed with its inhibitor, ICAD. Activation of caspase-3 causes the cleavage of ICAD, releasing CAD to cleave chromosomal DNA. ICAD has a molecular weight of approximately ~45 kDa in SDS-PAGE. The antibody recognizes the full length and cleaved form (~18 kDa) of mouse ICAD. A peptide corresponding to amino acids 312 to 331 of mouse ICAD was used as immunogen.
Molecular Weight 45 kDa
Application Notes Applications include western blot analysis (1:1000-1:4000). Mouse lung lysate is recommended as a positive control.
Comment

Related Products: ABIN967390

Restrictions For Research Use only
Format Liquid
Buffer Aqueous buffered solution.
Preservative Sodium azide
Storage 4 °C
Product cited in: Liu, Zou, Slaughter et al.: "DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis." in: Cell, Vol. 89, Issue 2, pp. 175-84, 1997 (PubMed).

Enari, Sakahira, Yokoyama et al.: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998 (PubMed).

Sakahira, Enari, Nagata: "Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis." in: Nature, Vol. 391, Issue 6662, pp. 96-9, 1998 (PubMed).

Sakahira, Enari, Nagata: "Functional differences of two forms of the inhibitor of caspase-activated DNase, ICAD-L, and ICAD-S." in: The Journal of biological chemistry, Vol. 274, Issue 22, pp. 15740-4, 1999 (PubMed).

Alternatives for antigen "DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA)", type "Antibodies"
Hosts (158), (24), (1)
Reactivities (125), (76), (22), (1), (1)
Applications (157), (59), (26), (24), (19), (14), (13), (10), (5), (4), (3), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (40), (29), (12), (12), (7), (6), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1)
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