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DFFA antibody (DNA Fragmentation Factor, 45kDa, alpha Polypeptide) (AA 312-331)

Details for Product anti-DFFA Antibody No. ABIN967295, Supplier: Log in to see
Antigen
  • DFFA
  • DFF-45
  • DFF1
  • ICAD
  • A330085O09Rik
  • DFF35
  • Dff45
  • ICAD-L
  • ICAD-S
Alternatives
anti-Mouse (Murine) DFFA antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
AA 312-331
38
28
18
16
13
12
11
9
5
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
158
72
31
1
1
Host
Rabbit
183
33
2
Clonality
Polyclonal
Conjugate
This DFFA antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
178
72
40
24
24
20
11
11
6
4
4
3
2
1
1
1
Supplier
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Brand BD Pharmingen™
Immunogen Mouse ICAD peptide aa. 312-331
Isotype IgG
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography.
Alternative Name ICAD (DFFA Antibody Abstract)
Background Apoptosis is essential in maintaining normal cellular homeostasis and for animal development. Morphological as well as biochemical changes are a hallmark of apoptosis and include membrane blebbing, chromosomal condensation and DNA fragmentation. These changes are caused by a class of cysteine proteases called caspases, which, upon activation can cleave multiple cytoplasmic and nuclear substrates. Caspases, once activated by a proapoptotic signal can induce a DNase, designated DFF/CAD to cleave these nuclear substrates. In growing, non-apoptotic cells DFF/CAD is complexed with its inhibitor, ICAD. Activation of caspase-3 causes the cleavage of ICAD, releasing CAD to cleave chromosomal DNA. ICAD has a molecular weight of approximately ~45 kDa in SDS-PAGE. The antibody recognizes the full length and cleaved form (~18 kDa) of mouse ICAD. A peptide corresponding to amino acids 312 to 331 of mouse ICAD was used as immunogen.
Molecular Weight 45 kDa
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes Applications include western blot analysis (1:1000-1:4000). Mouse lung lysate is recommended as a positive control.
Comment

Related Products: ABIN967390

Restrictions For Research Use only
Format Liquid
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-DFFA antibody (DNA Fragmentation Factor, 45kDa, alpha Polypeptide) (AA 312-331) (ABIN967295) Western blot analysis of ICAD (CT). Lysate from mouse lung lysate were probed with an...
Western Blotting (WB) image for anti-DFFA antibody (DNA Fragmentation Factor, 45kDa, alpha Polypeptide) (AA 312-331) (ABIN967295) anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 312-331) antibody (Image 2)
Product cited in: Sakahira, Enari, Nagata: "Functional differences of two forms of the inhibitor of caspase-activated DNase, ICAD-L, and ICAD-S." in: The Journal of biological chemistry, Vol. 274, Issue 22, pp. 15740-4, 1999 (PubMed).

Sakahira, Enari, Nagata: "Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis." in: Nature, Vol. 391, Issue 6662, pp. 96-9, 1998 (PubMed).

Enari, Sakahira, Yokoyama et al.: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998 (PubMed).

Liu, Zou, Slaughter et al.: "DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis." in: Cell, Vol. 89, Issue 2, pp. 175-84, 1997 (PubMed).