Immunoglobulin kappa Variable 1-27 (IGKV1-27) antibody

Antigen: Immunoglobulin kappa Variable 1-27 (IGKV1-27)

Clonality: Monoclonal (E5-1619)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN967297

Additional Information

Alternative name: A20

Immunogen: Human A20

Format: Liquid

Isotype: IgG1

Clone: E5-1619

Description: A20 was originally identified as a zinc finger protein, containing multiple Cys2/Cys2 finger motifs, induced by tumor necrosis factor alpha (TNFalpha). Further research has shown that A20 can negatively regulate NF-kappaB gene expression. NF-kappaB is an ubiquitously expressed transcription factor that regulates many genes involved in activation of inflammatory and cytokine responses. TNFalpha induces the activation of NF-kappaB by first recruiting a number of proteins to the TNF receptor. RIP and TRADD are two effector proteins which bind to the intracellular portion of the TNF receptor and form a multiprotein complex. Additional proteins, such as TRAF2 can interact with members of this complex. Although the exact mechanism of A20 regulation is unknown, it is believed that A20 interferes with either a TNF-induced RIP or TRAF2 mediated signaling pathway to block NF-kappaB expression. A20 mRNA is highly expressed in lymphoid tissues. A20 migrates in SDS/PAGE at ~80 kDa, which is equivalent to the predicted molecular weight. The antibody recognizes human A20. A fusion protein corresponding to the full length of human A20 was used as the immunogen.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Restrictions: For Research Use only

Images

Western blot analysis of A20. Lysate from 293 cells transfected with A20 was probed with anti-A20 (clone E5-1619) at concentrations of 0.25 (lane 1), 0.125 (lane 2), and 0.063 µg/ml (lane 3). A20 is identified as a band of ~80 kDa.

Publications

Product: Opipari, Boguski, Dixit: "The A20 cDNA induced by tumor necrosis factor alpha encodes a novel type of zinc finger protein." in: The Journal of biological chemistry, Vol. 265, Issue 25, pp. 14705-8, 1990 (PubMed).

Dixit, Green, Sarma et al.: "Tumor necrosis factor-alpha induction of novel gene products in human endothelial cells including a macrophage-specific chemotaxin." in: The Journal of biological chemistry, Vol. 265, Issue 5, pp. 2973-8, 1990 (PubMed).

Tewari, Wolf, Seldin et al.: "Lymphoid expression and regulation of A20, an inhibitor of programmed cell death." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 4, pp. 1699-706, 1995 (PubMed).

Wissing, Mouritzen, Jäättelä: "TNF-induced mitochondrial changes and activation of apoptotic proteases are inhibited by A20." in: Free radical biology & medicine, Vol. 25, Issue 1, pp. 57-65, 1999 (PubMed).

Heyninck, De Valck, Vanden Berghe et al.: "The zinc finger protein A20 inhibits TNF-induced NF-kappaB-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal and directly binds to a novel NF-kappaB-inhibiting protein ABIN." in: The Journal of cell biology, Vol. 145, Issue 7, pp. 1471-82, 1999 (PubMed).