Ubiquitin antibody

Details for Product No. ABIN967298
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Antigen
Synonyms
CG11624, DmUb, DmUbi-p63E, Dmel\\CG11624, Ub, Ubi-P63e, Ubi-p, Ubi63E, Ubi63p, Ubip, Ubiq, Ubp, ubi, ubi-p63E, ubi63E, CG18282, CG32744, CR32744, DmUbi-p5E, Dmel\\CG32744, ubiquitin, si:dz180g5.5, si: ... show more
CG11624, DmUb, DmUbi-p63E, Dmel\\CG11624, Ub, Ubi-P63e, Ubi-p, Ubi63E, Ubi63p, Ubip, Ubiq, Ubp, ubi, ubi-p63E, ubi63E, CG18282, CG32744, CR32744, DmUbi-p5E, Dmel\\CG32744, ubiquitin, si:dz180g5.5, si:busm1-180g5.5, LOC100194618, UBIQ, Ubiquitin, LOC100282068 show less
Reactivity
Human
(136), (72), (52), (51), (17), (11), (11), (9), (7), (7), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Host
Mouse
(121), (87), (3), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(5), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(147), (87), (81), (44), (39), (37), (22), (21), (12), (10), (3), (2), (1)
Pubmed 3 references available
Catalog no. ABIN967298
Quantity 100 µL
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Clone 6C1-17
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purity Ascites
Background Eukaryotic cells have evolved several pathways to degrade unwanted proteins. One of the best-understood of these pathways is the ubiquitin-mediated pathway. Ubiquitin is a small (8.5 kDa), highly conserved protein that covalently modifies cellular proteins and selectively targets these proteins for degradation by the 26S ATP-dependent proteasome. Proteins that have been modified by addition of a multiubiquitin chain are preferred substrates of the proteasome. However, ubiquitination does not serve exclusively to mark proteins for degradation. The ubiquitin proteolytic system plays an important role in many cellular processes including regulation of cell cycle, modulation of the immune and inflammatory responses, control of signal transduction pathways, development and differentiation. Furthermore, abnormalities in the ubiquitinmediated pathway have been shown to cause pathological disorders and in some cases lead to unregulated cell growth.
The clone 6C1.17 antibody is being provided as an ascites preparation that recognizes human ubiquitinated proteins, but should also recognize other species because of the highly conserved nature of this molecule. Bovine erythrocyte ubiquitin was coupled to ovalbumin with glutaraldehyde and used as the immunogen.
Research Area Neurology, Proteolysis / Ubiquitin, Alzheimer's Disease, Proteases, Amino Acids
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Buffer Aqueous buffered solution.
Preservative Sodium azide
Storage 4 °C
Supplier Images
anti-Ubiquitin antibody Western blot analysis of Ubiquitin. Human HL-60 cells (ATCC CCL-240) were either left untreated (lanes 1, 3, 5) or treated (2, 4, 6) with lactacystin (a proteosome inhibitor and causes ubiquitinated proteins to accumulate) (20 mM, 4 hr). Lysate from these cells was probed with anti-ubiquitin at dilutions of 1:2000 (lanes 1, 2), 1:4000 (lanes 3, 4), and 1:8000 (lanes 5, 6). Ubiquitinated proteins are identified at ~>200kDa.
Product cited in: Hershko, Ciechanover: "The ubiquitin system." in: Annual review of biochemistry, Vol. 67, pp. 425-79, 1998 (PubMed).

Chen, Smith, Accavitti-Loper et al.: "Ubiquitylation and destruction of endogenous c-mycS by the proteasome: are myc boxes dispensable?" in: Archives of biochemistry and biophysics, Vol. 374, Issue 2, pp. 306-12, 2000 (PubMed).

Smith, Chen, Reyland et al.: "Activation of atypical protein kinase C zeta by caspase processing and degradation by the ubiquitin-proteasome system." in: The Journal of biological chemistry, Vol. 275, Issue 51, pp. 40620-7, 2001 (PubMed).

Hosts (121), (87), (3), (2), (1)
Reactivities (136), (72), (52), (51), (17), (11), (11), (9), (7), (7), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Applications (147), (87), (81), (44), (39), (37), (22), (21), (12), (10), (3), (2), (1)
Conjugates (5), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (7), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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