You are viewing an incomplete version of our website. Please click to reload the website as full version.

Interferon Regulatory Factor 3 (IRF3) antibody

Details for Product No. ABIN967306, Supplier: Login to see New
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Synonyms irf3, IRF3, C920001K05Rik, IRF-3
Show less
(252), (85), (44), (6), (5), (3), (2)
(217), (59), (2)
Clonality (Clone)
Monoclonal ()
(4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Western Blotting (WB)
(236), (99), (62), (56), (39), (38), (31), (24), (11), (11), (9), (4), (3), (3), (2), (1), (1), (1), (1), (1)
Pubmed 8 references available
Supplier Login to see New
Catalog number from supplier Login to see New
Quantity 50 μg
Shipping to United States ( )
Brand BD Pharmingen™
Immunogen Human IRF-3 recombinant fusion protein
Clone SL-14-2
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name IRF-3 (IRF3 Antibody Abstract)
Background Viral infection in mammals can lead to the induction of multiple pathways as part of the host defense mechanism. One of the major pathways activated is the JAK-STAT pathway by various interferons (IFNalpha and IFNbeta). These IFNs exert their influence via transcriptional activation of specific target genes involved in anti-viral defense, for example the chemokine ISG15 gene or the major histocompatibility complex class I and II molecules. These genes in turn are regulated by the JAK-STAT signaling pathway and through interferon regulatory factors (IRFs). IRFs are a family of transcription factors which possess a broad range of activities. IRF-3 is one of nine members which all share a common DNA binding domain which binds to IFN stimulated response element (ISRE) found in the majority of IFN-inducible promoters. The IRF-3 gene expresses a 50 kDa protein which is constitutively expressed in all tissues. The protein undergoes post-translational modification as well as dimerization and is translocated from the cytoplasm to the nucleus upon viral infection or exposure to dsRNA. The antibody recognizes human IRF-3. A purified recombinant GST-IRF-3 fusion protein corresponding to human IRF-3 was used as the immunogen.
Molecular Weight 50-55 kDa
Pathways TLR Signaling
Application Notes Applications include western blot analysis (1.0-4.0 µg/ml). Store the antibody at -20°C. The antibody will only recognize the cytoplasmic form of IRF-3 and can not block IRF-3 DNA binding, although this application has not been tested. Jurkat control lysate [50 µg (1 µg/µl)] is provided as a western blot positive control (store lysate at -20°C). Additional control lysate (ABIN968537) is sold separately.

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Interferon Regulatory Factor 3 (IRF3) antibody (ABIN967306) Western blot analysis of IRF-3. Lysates from Jurkat cells were probed with anti-human...
Product cited in: Mamane, Heylbroeck, Génin et al.: "Interferon regulatory factors: the next generation." in: Gene, Vol. 237, Issue 1, pp. 1-14, 1999 (PubMed).

Hiscott, Pitha, Genin et al.: "Triggering the interferon response: the role of IRF-3 transcription factor." in: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, Vol. 19, Issue 1, pp. 1-13, 1999 (PubMed).

Wathelet, Lin, Parekh et al.: "Virus infection induces the assembly of coordinately activated transcription factors on the IFN-beta enhancer in vivo." in: Molecular cell, Vol. 1, Issue 4, pp. 507-18, 1998 (PubMed).

Ronco, Karpova, Vidal et al.: "Human papillomavirus 16 E6 oncoprotein binds to interferon regulatory factor-3 and inhibits its transcriptional activity." in: Genes & development, Vol. 12, Issue 13, pp. 2061-72, 1998 (PubMed).

Au, Moore, Lowther et al.: "Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 25, pp. 11657-61, 1996 (PubMed).

Darnell, Kerr, Stark: "Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins." in: Science (New York, N.Y.), Vol. 264, Issue 5164, pp. 1415-21, 1994 (PubMed).

Reich, Evans, Levy et al.: "Interferon-induced transcription of a gene encoding a 15-kDa protein depends on an upstream enhancer element." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 18, pp. 6394-8, 1987 (PubMed).

Loh, Chang, Fodor et al.: "Dissection of the interferon gamma-MHC class II signal transduction pathway reveals that type I and type II interferon systems share common signalling component(s)." in: The EMBO journal, Vol. 11, Issue 4, pp. 1351-63, 1992 (PubMed).

Catalog No. ABIN967306
Contact our Customer Service for availability and price in your country.

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)