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BCL2 antibody (B-Cell CLL/lymphoma 2)

Details for Product anti-BCL2 Antibody No. ABIN967311, Supplier: Log in to see
Antigen
  • AW986256
  • bcl-2
  • BCL-2
  • Bcl-2
  • Bcl2
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • PPP1R50
Alternatives
anti-Human BCL2 antibody for Immunohistochemistry (Frozen Sections)
Reactivity
Human
673
200
151
17
9
7
4
3
2
2
1
1
Host
Hamster
494
240
19
5
2
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This BCL2 antibody is un-conjugated
38
33
28
24
19
14
11
10
7
6
6
6
6
6
6
3
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
480
275
241
144
137
137
113
67
49
42
32
11
10
10
8
8
5
1
1
1
1
1
Supplier
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Brand BD Pharmingen™
Immunogen Human Bcl-2 Recombinant Protein
Clone 6C8
Isotype IgG
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background Bcl-2 is considered to be novel among proto-oncogenes because it blocks apoptosis (programmed cell death) in many cell types. Apoptosis is an active form of cellular suicide that typically requires new RNA and protein synthesis and is associated with distinct morphological changes including cell shrinkage, cytoplasm membrane blebbing, nuclear fragmentation and DNA degradation. The Bcl-2 gene was first found in t(14:18) containing follicular B-cell lymphomas. A high proportion of these lymphomas contains t(14:18) chromosomal translocations involving the human Bcl-2 gene. Translocation of Bcl-2 sequences from chromosome 18 onto the transcriptionally active immunoglobulin locus at chromosome band 14q32 in B-cells deregulates Bcl-2 gene expression, resulting in high levels of Bcl-2 mRNA and protein expression. Because Bcl-2 blocks apoptosis it may contribute to tumorigenesis by prolonged cell survival rather than by accelerating the rate of cell proliferation. The reduced molecular weight of Bcl-2 is 26 kDa. Additional minor bands at 27-31 kDa and 18-21 kDa may also be observed.
Molecular Weight 26 kDa
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis
Comment

Related Products: ABIN968537

Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store both the hamster anti-human Bcl-2 antibody and the Jurkat cell lysate undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (ABIN967311) Western blot analysis of Bcl-2. A Jurkat cell lysate (Human T-cell leukemia, ATCC TIB...
Background publications Núñez, Merino, Grillot, González-García: "Bcl-2 and Bcl-x: regulatory switches for lymphoid death and survival." in: Immunology today, Vol. 15, Issue 12, pp. 582-8, 1995

Yang, Zha, Jockel, Boise, Thompson, Korsmeyer: "Bad, a heterodimeric partner for Bcl-XL and Bcl-2, displaces Bax and promotes cell death." in: Cell, Vol. 80, Issue 2, pp. 285-91, 1995

Chiou, Rao, White: "Bcl-2 blocks p53-dependent apoptosis." in: Molecular and cellular biology, Vol. 14, Issue 4, pp. 2556-63, 1994

Nguyen, Millar, Yong, Korsmeyer, Shore: "Targeting of Bcl-2 to the mitochondrial outer membrane by a COOH-terminal signal anchor sequence." in: The Journal of biological chemistry, Vol. 268, Issue 34, pp. 25265-8, 1994

Yin, Oltvai, Korsmeyer: "BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax." in: Nature, Vol. 369, Issue 6478, pp. 321-3, 1994

Batistatou, Merry, Korsmeyer, Greene: "Bcl-2 affects survival but not neuronal differentiation of PC12 cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 13, Issue 10, pp. 4422-8, 1993

Hockenbery, Oltvai, Yin, Milliman, Korsmeyer: "Bcl-2 functions in an antioxidant pathway to prevent apoptosis." in: Cell, Vol. 75, Issue 2, pp. 241-51, 1993

Martin, Veis, Korsmeyer, Sugden: "Latent membrane protein of Epstein-Barr virus induces cellular phenotypes independently of expression of Bcl-2." in: Journal of virology, Vol. 67, Issue 9, pp. 5269-78, 1993

Oltvai, Milliman, Korsmeyer: "Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death." in: Cell, Vol. 74, Issue 4, pp. 609-19, 1993

Veis, Sentman, Bach, Korsmeyer: "Expression of the Bcl-2 protein in murine and human thymocytes and in peripheral T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 5, pp. 2546-54, 1993

Hockenbery, Nuñez, Milliman, Schreiber, Korsmeyer: "Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death." in: Nature, Vol. 348, Issue 6299, pp. 334-6, 1991

Hockenbery, Zutter, Hickey, Nahm, Korsmeyer: "BCL2 protein is topographically restricted in tissues characterized by apoptotic cell death." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 16, pp. 6961-5, 1991

McDonnell, Nunez, Platt, Hockenberry, London, McKearn, Korsmeyer: "Deregulated Bcl-2-immunoglobulin transgene expands a resting but responsive immunoglobulin M and D-expressing B-cell population." in: Molecular and cellular biology, Vol. 10, Issue 5, pp. 1901-7, 1990