CD93 Molecule (CD93) antibody

Details for Product No. ABIN967313
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Antigen
Synonyms C1QR1, C1qRP, CDw93, ECSM3, MXRA4, C1qR(P), dJ737E23.1, 6030404G09Rik, AA145088, AA4.1, AW555904, C1qr1, C1qrp, Ly68, C1qRp
Reactivity
Human
(58), (52), (30), (17), (12), (12)
Host
Mouse
(30), (28), (22)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(11), (6), (6), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Western Blotting (WB), Neutralization (Neut)
(56), (20), (16), (16), (11), (9), (7), (7), (1), (1), (1)
Pubmed 8 references available
Catalog no. ABIN967313
Quantity 0.1 mg
Price
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Clone R139
Isotype IgG2b, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name CDw93
Background The immunogen used to raise R139 was a preparation of CDw93 protein. CDw93 is also known as C1q-binding protein. Human C1qRp is a 631 amino acid protein (~66.5 kD) protein that is highly expressed on monocytes/macrophages, neutrophil granulocytes but not on T and B lymphocytes. C1qRp binds C1q, the recognition subunit of the first component (C1) of the complement pathway, as well as MBL (Mannose-binding-lectin) and SPA (Pulmonary Surfactant Protein A). Human C1qRp is involved in the C1q-mediated enhancement of phagocytosis. R139 is suitable to detect C1qRp expression on cells of myeloid lineage by flow cytometry, C1qRp in cellular lysates by Western blotting or immunoprecipitation. In addition, R139 neutralizes C1q-mediated enhancement of phagocytosis, as reported. CDw93 (C1qRp) has been reported to define a human stem cell population with hematopoietic and hepatic potential.
Synonyms: C1qRp
Application Notes Immunofluorescent Staining and Flow Cytometric Analysis:
The staining technique and blocking controls are described in detail by C. Prussin and D. Metcalfe. A suitable mouse IgG2b isotype control for assessing the level of background staining on human cells is recommended.

Western Blotting and Immunoprecipitation:
When run under non-reducing conditions, C1qRp migrates as a 100 kDa protein, due to high levels of glycosylation C1qR migrates as 126 kDa under reducing conditions. The R139 antibody is suitable to detect C1qRp in western blots and by immunprecipitation as described in the literature. Reactivity of the antibody with the reduced protein is dramatically decreased.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Storage 4 °C
Supplier Images
anti-CD93 Molecule (CD93) antibody Expression of C1qRp by unstimulated human peripheral blood mononuclear cells (PBMC). Human PBMC were stained with mouse anti-human C1qRp antibody (ABIN967313). A histogram overlay shows specific cell staining of gated CD14 positive cells with R139 followed by biotin anti-mouse secondary antibody and streptavidin-PE compared to isotype control.
General Guan, Burgess, Robinson et al.: "Phagocytic cell molecules that bind the collagen-like region of C1q. Involvement in the C1q-mediated enhancement of phagocytosis." in: The Journal of biological chemistry, Vol. 266, Issue 30, pp. 20345-55, 1991 (PubMed).

Guan, Robinson, Goodman et al.: "Cell-surface protein identified on phagocytic cells modulates the C1q-mediated enhancement of phagocytosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 8, pp. 4005-16, 1994 (PubMed).

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996 (PubMed).

Nepomuceno, Henschen-Edman, Burgess et al.: "cDNA cloning and primary structure analysis of C1qR(P), the human C1q/MBL/SPA receptor that mediates enhanced phagocytosis in vitro." in: Immunity, Vol. 6, Issue 2, pp. 119-29, 1997 (PubMed).

Nepomuceno, Tenner: "C1qRP, the C1q receptor that enhances phagocytosis, is detected specifically in human cells of myeloid lineage, endothelial cells, and platelets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 4, pp. 1929-35, 1998 (PubMed).

Tenner: "C1q receptors: regulating specific functions of phagocytic cells." in: Immunobiology, Vol. 199, Issue 2, pp. 250-64, 1999 (PubMed).

Nepomuceno, Ruiz, Park et al.: "C1qRP is a heavily O-glycosylated cell surface protein involved in the regulation of phagocytic activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 6, pp. 3583-9, 1999 (PubMed).

Danet, Luongo, Butler et al.: "C1qRp defines a new human stem cell population with hematopoietic and hepatic potential." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 16, pp. 10441-5, 2002 (PubMed).

Hosts (30), (28), (22)
Reactivities (58), (52), (30), (17), (12), (12)
Applications (56), (20), (16), (16), (11), (9), (7), (7), (1), (1), (1)
Conjugates (11), (6), (6), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Epitopes (3), (1)
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