B-Cell CLL/lymphoma 2 (BCL2) antibody

Details for Product No. ABIN967318
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Antigen
Synonyms AW986256, Bcl-2, C430015F12Rik, D630044D05Rik, D830018M01Rik, PPP1R50, BCL-2, bcl-2, Bcl2
Reactivity
Human
(444), (159), (125), (54), (36), (36), (14), (12), (3), (2), (2)
Host
Mouse
(366), (123), (8), (7), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(22), (17), (14), (12), (9), (7), (7), (7), (7), (7), (7), (7), (7), (6), (1), (1), (1)
Application
Western Blotting (WB), Immunoprecipitation (IP), Immunoelectron Microscopy (IEM)
(331), (179), (139), (118), (89), (84), (70), (56), (55), (43), (24), (10), (5), (2), (2), (2), (2), (1), (1)
Pubmed 10 references available
Quantity 150 µg
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Catalog No. ABIN967318
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Immunogen Human Bcl-2
Clone 4D7
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Components 1) 51-1483GR: Purified Mouse Anti-Human Bcl-2.
Quantity: 50 µg (3 ea).
Immunogen: Human Bcl-2 aa. 61-76.
Isotype: Mouse IgG1.
Molecular Weight: 26 kDa.
Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and Less or equal than 0.09% sodium azide.

2) 51-16526N: Jurkat Cell Lysate.
Quantity: 50 µg (1 ea).
Concentration: 1.0 mg/ml.
Storage Buffer: SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b-mercaptoethanol, 0.003% bromophenol blue, 5% glycerol).
Alternative Name Bcl-2
Background The bcl-2 gene was first discovered in t(14:18) follicular lymphomas. Translocation of bcl-2 sequences from chromosome 18 onto the transcriptionally active immunoglobulin locus at chromosome band 14q32 in B-cells deregulates bcl-2 gene expression, resulting in high levels of bcl-2 mRNA. Bcl-2 protein blocks apoptosis (programmed cell death), and thereby may contribute to tomorigenesis by prolonging cell survival rather than by accelerating the rate of cell proliferation. Abundant evidence implicates this protein in the suppression of apoptosis in many types of cells. Bcl-2 is an intracellular membrane protein and resides primarily in the nuclear envelope, outer mitochondrial membrane and endoplasmic reticulum. The reduced molecular weight of Bcl-2 is 26 kDa. Clone 4D7 recognizes human Bcl-2. A synthetic peptide containing amino acids 61-76 of the human Bcl-2 protein was used as immunogen. This peptide sequence differs from that of mouse Bcl-2 and hence 4D7 does not cross-react with mouse. 4D7 recognizes Bcl-2 as a 26 kDa band, however, additional higher molecular weight bands have been observed in some cell types. These bands may represent oligomers of Bcl-2, which have been reported to occur with members of the Bcl-2 family during the preparation of nonionic detergent extracts.
Molecular Weight 26 kDa
Research Area Cancer, Apoptosis/Necrosis
Application Notes Jurkat control lysate [50 µg (1 µg/µl)] is provided as a western blot positive control (store lysate at -20°C). Additional control lysate (ABIN968537) is sold separately. For immunohistochemistry of paraffin-embedded sections, the monoclonal mouse anti-human Bcl-2 (clone Bcl-2/100, ABIN967322) or clone 7/Bcl-2, (ABIN968025) is recommended.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-B-Cell CLL/lymphoma 2 (BCL2) antibody Western blot analysis of bcl-2. Lysate from Jurkat cells was probed with anti-bcl-2 (clone 4D7) at concentrations of 1.0 (lane 1), 0.5 (lane 2), and 0.25 myg/ml (lane 3). Bcl-2 is identified as a band of 26 kDa.
General Reed, Tanaka, Cuddy et al.: "A strategy for generating monoclonal antibodies against recombinant baculovirus-produced proteins: application to the Bcl-2 oncoprotein." in: Analytical biochemistry, Vol. 205, Issue 1, pp. 70-6, 1992 (PubMed).

Reed, Meister, Tanaka et al.: "Differential expression of bcl2 protooncogene in neuroblastoma and other human tumor cell lines of neural origin." in: Cancer research, Vol. 51, Issue 24, pp. 6529-38, 1992 (PubMed).

Pezzella, Tse, Cordell et al.: "Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation." in: The American journal of pathology, Vol. 137, Issue 2, pp. 225-32, 1990 (PubMed).

Reed, Tsujimoto, Alpers et al.: "Regulation of bcl-2 proto-oncogene expression during normal human lymphocyte proliferation." in: Science (New York, N.Y.), Vol. 236, Issue 4806, pp. 1295-9, 1987 (PubMed).

Tsujimoto, Cossman, Jaffe et al.: "Involvement of the bcl-2 gene in human follicular lymphoma." in: Science (New York, N.Y.), Vol. 228, Issue 4706, pp. 1440-3, 1985 (PubMed).

Teixeira, Reed, Pratt: "Estrogen promotes chemotherapeutic drug resistance by a mechanism involving Bcl-2 proto-oncogene expression in human breast cancer cells." in: Cancer research, Vol. 55, Issue 17, pp. 3902-7, 1995 (PubMed).

Wang, Millan, Cox et al.: "R-Ras promotes apoptosis caused by growth factor deprivation via a Bcl-2 suppressible mechanism." in: The Journal of cell biology, Vol. 129, Issue 4, pp. 1103-14, 1995 (PubMed).

Krajewski, Tanaka, Takayama et al.: "Investigation of the subcellular distribution of the bcl-2 oncoprotein: residence in the nuclear envelope, endoplasmic reticulum, and outer mitochondrial membranes." in: Cancer research, Vol. 53, Issue 19, pp. 4701-14, 1993 (PubMed).

Tanaka, Saito, Reed: "Structure-function analysis of the Bcl-2 oncoprotein. Addition of a heterologous transmembrane domain to portions of the Bcl-2 beta protein restores function as a regulator of cell survival." in: The Journal of biological chemistry, Vol. 268, Issue 15, pp. 10920-6, 1993 (PubMed).

Hsu, Youle: "Nonionic detergents induce dimerization among members of the Bcl-2 family." in: The Journal of biological chemistry, Vol. 272, Issue 21, pp. 13829-34, 1997 (PubMed).

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