BCL2 antibody (B-Cell CLL/lymphoma 2) (AA 61-76)

Details for Product anti-BCL2 Antibody No. ABIN967318, Supplier: Log in to see
Antigen
  • Bcl-2
  • PPP1R50
  • bcl-2
  • AW986256
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • BCL-2
  • Bcl2
  • B-cell CLL/lymphoma 2
  • B cell leukemia/lymphoma 2
  • B-cell leukemia/lymphoma 2
  • B-cell leukemia/lymphoma 2-like
  • B-cell CLL/lymphoma 2 protein
  • BCL2
  • Bcl2
  • LOC100224888
  • bcl2
  • LOC100359045
  • BCL-2
Alternatives
anti-Human BCL2 antibody for Flow Cytometry
Epitope
AA 61-76
164
76
65
62
44
33
25
25
23
21
18
15
15
15
13
13
13
11
9
9
6
6
5
5
5
5
5
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1032
257
212
54
28
22
18
18
5
4
4
3
2
2
2
2
Host
Mouse
562
535
19
6
2
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This BCL2 antibody is un-conjugated
50
49
44
38
25
24
22
16
15
14
14
14
14
14
14
14
13
13
13
7
7
7
6
6
6
6
6
6
6
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
814
380
375
329
324
323
227
192
169
81
40
15
14
13
12
11
9
4
3
2
1
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Brand BD Pharmingen™
Immunogen Human Bcl-2 aa. 61-76
Clone 4D7
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background The bcl-2 gene was first discovered in t(14:18) follicular lymphomas. Translocation of bcl-2 sequences from chromosome 18 onto the transcriptionally active immunoglobulin locus at chromosome band 14q32 in B-cells deregulates bcl-2 gene expression, resulting in high levels of bcl-2 mRNA. Bcl-2 protein blocks apoptosis (programmed cell death), and thereby may contribute to tomorigenesis by prolonging cell survival rather than by accelerating the rate of cell proliferation. Abundant evidence implicates this protein in the suppression of apoptosis in many types of cells. Bcl-2 is an intracellular membrane protein and resides primarily in the nuclear envelope, outer mitochondrial membrane and endoplasmic reticulum. The reduced molecular weight of Bcl-2 is 26 kDa. Clone 4D7 recognizes human Bcl-2. A synthetic peptide containing amino acids 61-76 of the human Bcl-2 protein was used as immunogen. This peptide sequence differs from that of mouse Bcl-2 and hence 4D7 does not cross-react with mouse. 4D7 recognizes Bcl-2 as a 26 kDa band, however, additional higher molecular weight bands have been observed in some cell types. These bands may represent oligomers of Bcl-2, which have been reported to occur with members of the Bcl-2 family during the preparation of nonionic detergent extracts.
Molecular Weight 26 kDa
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
Application Notes Jurkat control lysate [50 µg (1 µg/µl)] is provided as a western blot positive control (store lysate at -20°C). Additional control lysate (ABIN968537) is sold separately. For immunohistochemistry of paraffin-embedded sections, the monoclonal mouse anti-human Bcl-2 (clone Bcl-2/100, ABIN967322) or clone 7/Bcl-2, (ABIN968025) is recommended.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 61-76) antibody (ABIN967318) Western blot analysis of bcl-2. Lysate from Jurkat cells was probed with anti-bcl-2 (...
Background publications Hsu, Youle: "Nonionic detergents induce dimerization among members of the Bcl-2 family." in: The Journal of biological chemistry, Vol. 272, Issue 21, pp. 13829-34, 1997 (PubMed).

Teixeira, Reed, Pratt: "Estrogen promotes chemotherapeutic drug resistance by a mechanism involving Bcl-2 proto-oncogene expression in human breast cancer cells." in: Cancer research, Vol. 55, Issue 17, pp. 3902-7, 1995 (PubMed).

Wang, Millan, Cox, Der, Rapp, Beck, Zha, Reed: "R-Ras promotes apoptosis caused by growth factor deprivation via a Bcl-2 suppressible mechanism." in: The Journal of cell biology, Vol. 129, Issue 4, pp. 1103-14, 1995 (PubMed).

Krajewski, Tanaka, Takayama, Schibler, Fenton, Reed: "Investigation of the subcellular distribution of the bcl-2 oncoprotein: residence in the nuclear envelope, endoplasmic reticulum, and outer mitochondrial membranes." in: Cancer research, Vol. 53, Issue 19, pp. 4701-14, 1993 (PubMed).

Tanaka, Saito, Reed: "Structure-function analysis of the Bcl-2 oncoprotein. Addition of a heterologous transmembrane domain to portions of the Bcl-2 beta protein restores function as a regulator of cell survival." in: The Journal of biological chemistry, Vol. 268, Issue 15, pp. 10920-6, 1993 (PubMed).

Reed, Tanaka, Cuddy, Cho, Smith, Kallen, Saragovi, Torigoe: "A strategy for generating monoclonal antibodies against recombinant baculovirus-produced proteins: application to the Bcl-2 oncoprotein." in: Analytical biochemistry, Vol. 205, Issue 1, pp. 70-6, 1992 (PubMed).

Pezzella, Tse, Cordell, Pulford, Gatter, Mason: "Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation." in: The American journal of pathology, Vol. 137, Issue 2, pp. 225-32, 1990 (PubMed).

Reed, Tsujimoto, Alpers, Croce, Nowell: "Regulation of bcl-2 proto-oncogene expression during normal human lymphocyte proliferation." in: Science (New York, N.Y.), Vol. 236, Issue 4806, pp. 1295-9, 1987 (PubMed).

Tsujimoto, Cossman, Jaffe, Croce: "Involvement of the bcl-2 gene in human follicular lymphoma." in: Science (New York, N.Y.), Vol. 228, Issue 4706, pp. 1440-3, 1985 (PubMed).

Product cited in: Reed, Meister, Tanaka, Cuddy, Yum, Geyer, Pleasure: "Differential expression of bcl2 protooncogene in neuroblastoma and other human tumor cell lines of neural origin." in: Cancer research, Vol. 51, Issue 24, pp. 6529-38, 1992 (PubMed).

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