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C-MYC antibody (V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian))

Details for Product anti-MYC Antibody No. ABIN967320, Supplier: Log in to see
Antigen
  • AU016757
  • bHLHe39
  • c-myc
  • c-Myc
  • C-MYC
  • cmyc
  • CMYC
  • CMyc
  • LOC100136746
  • mMyc
  • MRTL
  • MYC
  • Myc2
  • MYCC
  • Niard
  • Nird
  • RNCMYC
  • zc-myc
Alternatives
anti-Human C-MYC antibody for Flow Cytometry
Reactivity
Human
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Host
Mouse
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1
Clonality (Clone)
Monoclonal ()
Conjugate
This C-MYC antibody is un-conjugated
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4
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Application
Fluorescence Microscopy (FM), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Supplier
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Brand BD Pharmingen™
Immunogen Human c-Myc Peptide
Clone 9E10
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name c-Myc (MYC Antibody Abstract)
Background The myc gene family contains at least seven closely related genes, c-myc, N-myc, L-myc, P-myc, R-myc, S-myc, and B-myc. C-myc plays a role in proliferation, transformation, and differentiation. It is expressed during embryonic development, in a wide variety of adult tissues, and is amplified in many tumors, particularly lung, breast, cervical and colon carcinomas. Cellular localization has been described as nuclear and/or cytopasmic. C-myc has three sequence motifs in the carboxy terminus, a leucine zipper, a helix-loop-helix, and a basic region. It forms sequence-specific (CACGTG) DNA binding heterodimers with max, a helixloop-helix/leucine zipper protein. Both the leucine zipper domain and the helixloop-helix motif of c-myc contribute to heterodimer formation. DNA binding of myc-max dimers results in a conformational change of the DNA and transcriptional activation. C-myc migrates at 62 kDa in SDS-PAGE. Clone 9E10 recognizes human c-myc. A synthetic peptide corresponding to a C-terminal epitope of the human c-myc protein (AEEQKLISEEDL) was used as an immunogen.
Research Area Cancer, Cell Cycle, Transcription Factors
Pathways p53 Signaling, Cell Division Cycle
Application Notes It is particularly useful for western blot analysis of myc-tagged recombinant proteins, and recombinant c-myc. Jurkat control lysate [50 µg (1 µg/µl)] is provided as a western blot positive control (store lysate at -20 °C). Additional control lysate is sold separately.
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-C-MYC antibody (V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian)) (ABIN967320) Western blot analysis of c-myc. Lysate from Jurkat cells was probed with anti-c-myc (...
Product cited in: Liu, Hsu, Goeddel, Karin: "Dissection of TNF receptor 1 effector functions: JNK activation is not linked to apoptosis while NF-kappaB activation prevents cell death." in: Cell, Vol. 87, Issue 3, pp. 565-76, 1996 (PubMed).

Lukas, Parry, Aagaard, Mann, Bartkova, Strauss, Peters, Bartek: "Retinoblastoma-protein-dependent cell-cycle inhibition by the tumour suppressor p16." in: Nature, Vol. 375, Issue 6531, pp. 503-6, 1995 (PubMed).

Royds, Sharrard, Wagner, Polacarz: "Cellular localisation of c-myc product in human colorectal epithelial neoplasia." in: The Journal of pathology, Vol. 166, Issue 3, pp. 225-33, 1992 (PubMed).

Tosca, Linardopoulos, Malliri, Hatziolou, Nicolaidou, Spandidos: "Implication of the ras and myc oncoproteins in the pathogenesis of mycosis fungoides." in: Anticancer research, Vol. 11, Issue 4, pp. 1433-8, 1992 (PubMed).

Blackwood, Eisenman: "Max: a helix-loop-helix zipper protein that forms a sequence-specific DNA-binding complex with Myc." in: Science (New York, N.Y.), Vol. 251, Issue 4998, pp. 1211-7, 1991 (PubMed).

DePinho, Schreiber-Agus, Alt: "myc family oncogenes in the development of normal and neoplastic cells." in: Advances in cancer research, Vol. 57, pp. 1-46, 1991 (PubMed).

Gosney, Field, Gosney, Lye, Spandidos, Butt: "c-myc oncoprotein in bronchial carcinoma: expression in all major morphological types." in: Anticancer research, Vol. 10, Issue 3, pp. 623-8, 1990 (PubMed).

Karayiannis, Yiagnisis, Papadimitriou, Field, Spandidos: "Evaluation of the ras and myc oncoproteins in benign gastric lesions." in: Anticancer research, Vol. 10, Issue 5A, pp. 1127-33, 1990 (PubMed).

Spandidos, Yiagnisis, Papadimitriou, Field: "ras, c-myc and c-erbB-2 oncoproteins in human breast cancer." in: Anticancer research, Vol. 9, Issue 5, pp. 1385-93, 1990 (PubMed).

Evan, Lewis, Ramsay, Bishop: "Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product." in: Molecular and cellular biology, Vol. 5, Issue 12, pp. 3610-6, 1986 (PubMed).

Background publications Campbell, Kessler, Fambrough: "The alternative carboxyl termini of avian cardiac and brain sarcoplasmic reticulum/endoplasmic reticulum Ca(2+)-ATPases are on opposite sides of the membrane." in: The Journal of biological chemistry, Vol. 267, Issue 13, pp. 9321-5, 1992 (PubMed).

Williams, Piris, Wyllie: "Immunohistochemical demonstration of altered intracellular localization of the C-Myc oncogene product in human colorectal neoplasms." in: The Journal of pathology, Vol. 160, Issue 4, pp. 287-93, 1990 (PubMed).

Tiniakos, Spandidos, Kakkanas, Pintzas, Pollice, Tiniakos: "Expression of ras and myc oncogenes in human hepatocellular carcinoma and non-neoplastic liver tissues." in: Anticancer research, Vol. 9, Issue 3, pp. 715-21, 1989 (PubMed).