Alternatives Western Blotting (WB)
|4 references available|
|Quantity||50 µg (Variants)|
|Price||Product not available in this region.|
1) 51-6875GR: Purified Mouse Anti-Human ORC-2.
Quantity: 50 µg (1 ea).
Concentration: 0.25 mg/ml.
Immunogen: The N-terminal half of Hsorc2p.
Isotype: Mouse IgG1, kappa.
Molecular Weight: 72 kDa.
Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and Less or equal than 0.09% sodium azide.
2) 51-16516N: HeLa Control Lysate.
Quantity: 50 µg (1 ea).
Concentration: 1.0 mg/ml.
Storage Buffer: SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b-mercaptoethanol, 0.003% bromophenol blue, 5% glycerol).
|Description||DNA replication in eukaryotes takes place in multiple sites of origins of replication scattered throughout the genome. The origin recognition complex (ORC) is a multiprotein assemblage originally identified in S. cerevisiae budding yeast that binds to the ARS (autonomously replicating sequence) consensus, a DNA motif which is an essential part of yeast origins of replication. The ORC complex consists of six distinct polypeptides (ORC1-ORC6), all having similar homology and essential for viability. ORC binding has also been implicated in transcriptional silencing at certain yeast loci. Our current understanding of the initiation of DNA replication in eukaryotes is based on studies done in yeast, but recent work has identified ORC proteins in higher eukaryotes. Mammals have close protein homologs to the yeast ORC proteins which are believed to be essential components of the mammalian origin recognition complex and may play a widespread role in packaging chromosomal domains through interactions with heterochromatin-organizing factors. There is a striking preferential but not exclusive association of ORC2 with heterochromatin on interphase and mitotic chromosomes. In Drosophila, ORC2 is highly concentrated at foci along the apical surface of the interphase nuclei, consistent with the known localization of pericentric heterochromatin. Consistent with this localization, ORC1, ORC2, ORC5 and ORC6 are found in a complex. Additionally, ORC2 has been shown to co-immunoprecipitate with ORC4. ORC2 has a molecular weight of approximately 72 kDa in western blot analysis. The antibody recognizes human ORC2. The N-terminal half of Hsorc2p fused to GST was used as immunogen.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Related Products: ABIN967389, ABIN968535
|Application Notes||Applications include western blot analysis (0.25-1.0 µg/ml). Store the antibody at -20°C. HeLa control lysate [50 µg (1 µg/µl)] is provided as a western blot positive control (Component No. 51-16516N, store lysate at -20°C). Additional control lysate (ABIN968535) is sold separately.|
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Storage||Store undiluted at -20°C.|
|Research Area||Chromatin and Nuclear Signaling, DNA/RNA|
|Restrictions||For Research Use only|
|Western blot analysis of ORC2. Lysate from HeLa cells was probed with anti-ORC2 (clone 920-4-41, Component No. 51-6875GR) at concentrations of 1.0 (lane 1), 0.5 (lane 2), and 0.25 µg/ml (lane 3). ORC2 is identified as a band of 72 kDa.|
Gavin, Hidaka, Stillman: "Conserved initiator proteins in eukaryotes." in: Science (New York, N.Y.), Vol. 270, Issue 5242, pp. 1667-71, 1996 (PubMed).
Takahara, Bong, Brevard et al.: "Mouse and human homologues of the yeast origin of replication recognition complex subunit ORC2 and chromosomal localization of the cognate human gene ORC2L." in: Genomics, Vol. 31, Issue 1, pp. 119-22, 1996 (PubMed).
Quintana, Hou, Thome et al.: "Identification of HsORC4, a member of the human origin of replication recognition complex." in: The Journal of biological chemistry, Vol. 272, Issue 45, pp. 28247-51, 1997 (PubMed).
Pak, Pflumm, Chesnokov et al.: "Association of the origin recognition complex with heterochromatin and HP1 in higher eukaryotes." in: Cell, Vol. 91, Issue 3, pp. 311-23, 1997 (PubMed).
|Hosts||Rabbit (5), Rat (4), Mouse (3)|
|Reactivities||Human (11), Mouse (Murine) (3), Rat (Rattus) (3), Cat (Feline) (1), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1), Yeast (1)|
|Applications||Western Blotting (WB) (12), ELISA (7), Immunoprecipitation (IP) (6), Immunofluorescence (IF) (2), Immunocytochemistry (ICC) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1), Immunohistochemistry (IHC) (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1)|