Heat Shock 70kDa Protein 8 (HSPA8) antibody

Antigen: Heat Shock 70kDa Protein 8 (HSPA8)

Synonyms: LAP1, HSC54, HSC70, HSC71, HSP71, HSP73, NIP71, HSPA10, MGC29929, MGC131511

Clonality: Monoclonal (W27)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN967328

Additional Information

Alternative name: Hsp70/Hsc70

Immunogen: Human Hsp70/Hsc70 recombinant protein

Components: 1) 51-6892GR: Purified Mouse Anti-Human Hsp70/Hsc70.
Quantity: 50 µg (1 ea).
Concentration: 0.25 mg/ml.
Clone: W27.
Immunogen: Human Hsp70/Hsc70 recombinant protein.
Isotype: Mouse IgG2a.
Molecular Weight: 70 kDa.
Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and Less or equal than 0.09% sodium azide.

2) 51-16516N: HeLa Control Lysate.
Quantity: 50 µg (1 ea).
Concentration: 1.0 mg/ml.
Storage Buffer: SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b-mercaptoethanol, 0.003% bromophenol blue, 5% glycerol).

Format: Liquid

Isotype: IgG2a

Clone: W27

Description: Heat shock proteins (Hsp70/Hsc70) are a class of proteins which increase their rate of synthesis when exposed to heat or other cellular stresses. It is now apparent that these proteins also function under normal conditions to facilitate folding and/or refolding of proteins, and to prevent aggregation. Members of the Hsp70 family bind ATP, and function as molecular chaperones by assisting in the folding of other proteins in various intracellular compartments. All Hsp70/Hsc70 proteins consist of two domains: a highly conserved N-terminal ATP-ase domain (44 kDa) and a C-terminal region (25 kDa) which is composed of a substrate binding domain.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Molecular Weight: 70 kDa

Comments:

Related Products: ABIN968535, ABIN967389

Application Details

Concentration: 0.25 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store the purified mouse anti-human Hsp70/Hsc70 antibody undiluted at -20°C. Store the HeLa control lysate undiluted at -20°C.

Research Area: Heat Shock Proteins

Restrictions: For Research Use only

Images

Western blot analysis for Hsp70/Hsc70. A HeLa cell lysate (Human cervical epitheloid carcinoma, ATCC CCL-2) was probed with the mouse anti-human Hsp70/Hsc70 antibody at concentrations of 1.0 µg/mL (lane 1), 0.5 µg/mL (lane 2), and 0.25 µg/mL (lane 3). Hsp70/Hsc70 is identified as a protein of ~70 kDa.

Publications

Product: Minota, Cameron, Welch et al.: "Autoantibodies to the constitutive 73-kD member of the hsp70 family of heat shock proteins in systemic lupus erythematosus." in: The Journal of experimental medicine, Vol. 168, Issue 4, pp. 1475-80, 1988 (PubMed).

Hang, Fox: "Expression of hsp70 induced in CHO cells by 45.0 degrees C hyperthermia is cell cycle associated and DNA synthesis dependent." in: Cytometry, Vol. 19, Issue 2, pp. 119-25, 1995 (PubMed).

Bukau, Horwich: "The Hsp70 and Hsp60 chaperone machines." in: Cell, Vol. 92, Issue 3, pp. 351-66, 1998 (PubMed).

Pilon, Schekman: "Protein translocation: how Hsp70 pulls it off." in: Cell, Vol. 97, Issue 6, pp. 679-82, 1999 (PubMed).