Western blot analysis of caspase-8. Lysates from control (lanes 1-3) and camptothecin treated Jurkat cells (lanes 4-6) were probed with anti-human caspase-8 (clone 3-1-9, ABIN967335) at the following concentrations: 0.5 (lanes 1,4), 0.25 (lanes 2,5), and 0.125 µg/ml (lanes 3,6). Caspase-8 is identified as 55/50 kDa (proform), 40/36 kDa (cleaved), and 23 kDa (cleaved) bands in treated cells and the 55 kDa band in control cells.
Immunoprecipitation/western blot analysis of caspase-8. Lysates from control (lane 1) or camptothecin-treated Jurkat cells (lane 2) were each immunoprecipitated with anti-human caspase-8 (clone 3-1-9), and western blotted with anti-human caspase-8 (clone 3-1-9). The 55 kDa proform caspase-8 was identified in both control and camptothecin-treated cells.