You are viewing an incomplete version of our website. Please click to reload the website as full version.

Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (AA 1-134)

Details for Product anti-CASP9 Antibody No. ABIN967339, Supplier: Log in to see
Antigen
  • AI115399
  • APAF-3
  • APAF3
  • Apaf3
  • AW493809
  • CASP-9
  • Casp-9-CTD
  • CASP9
  • Casp9_v1
  • Caspase-9
  • Ice-Lap6
  • ICE-LAP6
  • MCH6
  • Mch6
  • PPP1R56
Alternatives
anti-Human Caspase 9 antibody for Western Blotting
Epitope
AA 1-134, N-Term
35
22
20
19
17
16
13
13
13
13
12
11
11
9
8
7
7
7
6
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
413
119
116
19
13
9
6
5
4
3
3
2
1
1
1
1
Host
Mouse
421
45
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Caspase 9 antibody is un-conjugated
11
10
7
4
4
4
4
4
4
4
4
4
3
3
3
Application
Western Blotting (WB)
390
161
128
78
69
68
45
34
13
13
8
6
2
2
2
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Brand BD Pharmingen™
Immunogen Human caspase-9 N-terminal fragment aa 1-134
Clone 2
Isotype IgG1, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Caspase-9 (CASP9 Antibody Abstract)
Background The caspase family of cysteine proteases plays a key role in apoptosis and inflammation. Caspases are synthesized as inactive proenzymes containing three domains, that are processed into large and small subunits that associate to form the active enzyme. Processing can occur in apoptotic cells by either transactivation, self-proteolysis or cleavage by another protease. While caspases share a common structure, there are some differences, such as the preferred substrate specificity. These sequence differences in specificity, as well as the size of the NH2 -terminal prodomain, can be used to catagorize the caspases into functional groups including apoptotic initiators (long prodomains), apoptotic executioners (short prodomains), and cytokine processors. Caspase-9 is a member of the apoptotic initiator group of caspases which include caspases-2, -8, and -10. Activation of caspase-9 occurs in the presence of cytochrome c, following an interaction between caspase-9 and APAF-1. Activation may also be triggered directly by the cytotoxic T-cell protease, granzyme B. Active caspase-9 cleaves and thus activates caspase-3, and is also a relevant target of active caspase-3. Caspase-9 can also cleave the nuclear protein PARP. Northern blot analysis suggests that high expression of caspase-9 is found in the heart, testis, and ovary. The antibody recognizes the 47 kDa proform and 37 kDa cleaved form of human caspase-9. The N-terminal fragment (amino acids 1-134) of human caspase-9 was used as an immunogen.
Synonyms: ICE-LAP-6, Mch6, Apaf-3
Molecular Weight 47, 37 kDa
Research Area Apoptosis/Necrosis, Cancer
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Applications include western blot analysis (0.5 - 1.0 µg/ml). Jurkat cells (ATCC TIB-152) are suggested as a positive control. Antibodies-online offers several caspase-9 antibodies. A Jurkat model cell system was used to evaluate these antibodies, these results are summarized in the following table. However, actual bands observed could vary according to the cell model system or treatment used.
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (AA 1-134) (ABIN967339) Western blot analysis of caspase-9. Lysates from control (lanes 1-3) and amptothecin ...
Product cited in: Wolf, Green: "Suicidal tendencies: apoptotic cell death by caspase family proteinases." in: The Journal of biological chemistry, Vol. 274, Issue 29, pp. 20049-52, 1999 (PubMed).

Cohen: "Caspases: the executioners of apoptosis." in: The Biochemical journal, Vol. 326 ( Pt 1), Issue 5693, pp. 1-16, 1997 (PubMed).

Li, Nijhawan, Budihardjo, Srinivasula, Ahmad, Alnemri, Wang: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997 (PubMed).

Thornberry, Rano, Peterson, Rasper, Timkey, Garcia-Calvo, Houtzager, Nordstrom, Roy, Vaillancourt, Chapman, Nicholson: "A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 29, pp. 17907-11, 1997 (PubMed).

Background publications Duan, Chinnaiyan, Hudson, Wing, He, Dixit: "ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis." in: The Journal of biological chemistry, Vol. 271, Issue 3, pp. 1621-5, 1996 (PubMed).

Srinivasula, Fernandes-Alnemri, Zangrilli, Robertson, Armstrong, Wang, Trapani, Tomaselli, Litwack, Alnemri: "The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32." in: The Journal of biological chemistry, Vol. 271, Issue 43, pp. 27099-106, 1996 (PubMed).