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Cyclin-Dependent Kinase 2 (CDK2) antibody

Details for Product No. ABIN967341
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Antigen
Synonyms p33(CDK2), A630093N05Rik, zgc:56598, zgc:77684, wu:fa10c02, eg1, MGC84068, cinp, CINP
Reactivity
Human
(167), (99), (92), (26), (15), (12), (12), (3), (3), (3), (2), (2), (1)
Host
Rabbit
(153), (25)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
Western Blotting (WB), Immunoprecipitation (IP)
(149), (70), (50), (40), (26), (20), (18), (15), (14), (9), (5), (2), (1), (1), (1), (1)
Pubmed 6 references available
Quantity 100 µL
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Catalog No. ABIN967341
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Isotype Ig
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
Purification Purified from antiserum by affinity chromatography.
Purity Serum
Components 1) 51-9000091: Polyclonal Rabbit Anti-Cdk2.
Quantity: 0.1 ml (1 ea).
Immunogen: Human Cdk2 aa. 287-298 Peptide.
Molecular Weight: 33 kDa.
Storage Buffer: Aqueous buffered solution containing Less or equal than 0.09% sodium azide.

2) 51-16516N: HeLa Control Lysate.
Quantity: 50 µg (1 ea).
Concentration: 1.0 mg/ml.
Storage Buffer: SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b-mercaptoethanol, 0.003% bromophenol blue, 5% glycerol).
Alternative Name Cdk2
Background Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell cycle control in eukaryotes. Cdks are catalytic subunits whose activity requires interaction with their regulatory subunits, the cyclins, as well as specific phosphorylation events. The precise timing of cyclin-cdk activity during the cell cycle determines whether the cell cycle continues or becomes blocked. Cdks 2, 4, 5, and 6 may associate with D-type cyclins. Interaction between cdk2 and cyclin E during G1/S transition creates a complex with histone H1 kinase activity. This complex is thought to be required for the initiation and progression of DNA replication during S phase. Cdk2-cyclin A complexes appear during late S phase and also play a role in progression of DNA replication. Substrates for cdk-cyclin complexes include nuclear lamins, histones, oncogenes, (c-src, c-abl, SV40 large-T), tumor suppressor genes (e.g., RB and p53), nucleolin, RNA polymerase II and others. A synthetic peptide corresponding to amino acids 287-298 (QDVTKPVPHLRL) at the C-terminus of human cdk2, with the addition of a C-terminal cysteine residue (C) to facilitate coupling to KLH, was used as immunogen to generate this antibody.
Molecular Weight 33 kDa
Research Area Cancer, Cell Cycle, Kinases/Phosphatases
Comment

Related Products: ABIN967390, ABIN968535

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Cyclin-Dependent Kinase 2 (CDK2) antibody Western blot analysis for Cdk2. A HeLa cell lysate (Human cervical epitheloid carcinoma, ATCC CCL-2) was probed with the polyclonal Rabbit Anti-Cdk2 antibody at a 1:1000 dilution (lane 1).
Product cited in: Zhang, Xiong, Beach: "Proliferating cell nuclear antigen and p21 are components of multiple cell cycle kinase complexes." in: Molecular biology of the cell, Vol. 4, Issue 9, pp. 897-906, 1994 (PubMed).

Peter, Herskowitz: "Joining the complex: cyclin-dependent kinase inhibitory proteins and the cell cycle." in: Cell, Vol. 79, Issue 2, pp. 181-4, 1994 (PubMed).

Sherr: "G1 phase progression: cycling on cue." in: Cell, Vol. 79, Issue 4, pp. 551-5, 1994 (PubMed).

Heichman, Roberts: "Rules to replicate by." in: Cell, Vol. 79, Issue 4, pp. 557-62, 1994 (PubMed).

Hunter, Pines: "Cyclins and cancer. II: Cyclin D and CDK inhibitors come of age." in: Cell, Vol. 79, Issue 4, pp. 573-82, 1994 (PubMed).

Xiong, Zhang, Beach: "Subunit rearrangement of the cyclin-dependent kinases is associated with cellular transformation." in: Genes & development, Vol. 7, Issue 8, pp. 1572-83, 1993 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN967341
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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