P21-Activated Kinase 4 (PAK4) antibody

Details for Product No. ABIN967342
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Antigen
Synonyms 5730488L07Rik, AW555722, mKIAA1142
Reactivity
Human
(113), (59), (50), (38), (37), (37), (13), (2)
Host
Mouse
(100), (16), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application
Western Blotting (WB)
(86), (46), (31), (30), (18), (8), (8), (3), (3), (1), (1)
Pubmed 2 references available
Quantity 50 μg
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Catalog No. ABIN967342
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Clone E40-883
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Components 1) 51-16516N: HeLa Control Lysate.
Quantity: 50 µg (1 ea).
Concentration: 1.0 mg/ml.
Clone: E40-883.
Immunogen: Human PAK4 recombinant fusion protein.
Isotype: Mouse IgG1, kappa.
Molecular Weight: 64 kDa.
Storage Buffer: SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b-mercaptoethanol, 0.003% bromophenol blue, 5% glycerol)

2) 51-8102KC: Purified Mouse Anti-Human PAK.
Quantity: 50 µg (1 ea).
Concentration: 0.5 mg/ml.
Storage Buffer: Aqueous buffered solution containing Less or equal than 0.09% sodium azide).
Alternative Name PAK4
Background P21/Rac/Cdc42 activated kinases (PAKs) are serine/threonine kinases which regulate morphological and cytoskeletal changes in a variety of cell types, and are also implicated in MAP kinase pathways. PAKs contain an N-terminal regulatory domain and a C-terminal catalytic domain, and become activated following proteolytic cleavage between these regions. The activity of PAKs has been shown to be regulated by the GTPases Rac and Cdc42.1 Several members of the PAK family have been identified and implicated in regulation of the cytoskeletal organization induced by Rac and Cdc42, however, the cytoskeletal organizational change can occur independently of the known PAKs. PAK4 has been shown to provide a direct link between Cdc42 activation and cytoskeleton organization. PAK4 consists of 591 amino acids with a predicted molecular weight of about 64 kDa. The antibody recognizes human PAK4. A purified recombinant 6xHis-tagged fusion protein corresponding to the full length of human PAK4 was used as the immunogen.
Research Area Cancer, Cell Cycle, Cytoskeleton, Microfilaments, Apoptosis/Necrosis, Kinases/Phosphatases
Application Notes HeLa control lysate [50 µg (1 µg/µl)] is provided as a positive control (store lysate at -20°C). Additional HeLa lysate (ABIN968535) is sold separately as a ready-to-use western blot control.
Comment

Related Products: ABIN968535, ABIN967389

Restrictions For Research Use only
Format Liquid
Storage 4 °C/-20 °C
Supplier Images
anti-P21-Activated Kinase 4 (PAK4) antibody Western blot analysis of PAK4. Lysate from HeLa cells was probed with anti-PAK4 (clone E40-883, ABIN967342) at concentrations of 2.0 (lane 1), 1.0 (lane 2), and 0.5 µg/ml (lane 3). PAK4 is identified as a band of ~64 kDa.
Product cited in: Knaus, Morris, Dong et al.: "Regulation of human leukocyte p21-activated kinases through G protein--coupled receptors." in: Science (New York, N.Y.), Vol. 269, Issue 5221, pp. 221-3, 1995 (PubMed).

Abo, Qu, Cammarano et al.: "PAK4, a novel effector for Cdc42Hs, is implicated in the reorganization of the actin cytoskeleton and in the formation of filopodia." in: The EMBO journal, Vol. 17, Issue 22, pp. 6527-40, 1999 (PubMed).

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