P21-Activated Kinase 4 (PAK4) antibody

Details for Product No. ABIN967342
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Synonyms 5730488L07Rik, AW555722, mKIAA1142
(157), (62), (56), (13), (3), (2), (2), (1)
(137), (22), (2)
Clonality (Clone)
Monoclonal ()
(9), (9), (8), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3)
Western Blotting (WB)
(122), (58), (45), (30), (18), (16), (7), (6), (5), (2), (1), (1)
Pubmed 2 references available
Quantity 50 μg
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Brand BD Pharmingen™
Immunogen Human PAK4 recombinant fusion protein
Clone E40-883
Isotype IgG1, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name PAK4 (PAK4 Antibody Abstract)
Background P21/Rac/Cdc42 activated kinases (PAKs) are serine/threonine kinases which regulate morphological and cytoskeletal changes in a variety of cell types, and are also implicated in MAP kinase pathways. PAKs contain an N-terminal regulatory domain and a C-terminal catalytic domain, and become activated following proteolytic cleavage between these regions. The activity of PAKs has been shown to be regulated by the GTPases Rac and Cdc42.1 Several members of the PAK family have been identified and implicated in regulation of the cytoskeletal organization induced by Rac and Cdc42, however, the cytoskeletal organizational change can occur independently of the known PAKs. PAK4 has been shown to provide a direct link between Cdc42 activation and cytoskeleton organization. PAK4 consists of 591 amino acids with a predicted molecular weight of about 64 kDa. The antibody recognizes human PAK4. A purified recombinant 6xHis-tagged fusion protein corresponding to the full length of human PAK4 was used as the immunogen.
Molecular Weight 64 kDa Component: 51-16516N HeLa Control Lysate
Research Area Cancer, Cell Cycle, Cytoskeleton, Microfilaments, Apoptosis/Necrosis, Kinases/Phosphatases
Pathways RTK signaling
Application Notes HeLa control lysate [50 µg (1 µg/µl)] is provided as a positive control (store lysate at -20°C). Additional HeLa lysate (ABIN968535) is sold separately as a ready-to-use western blot control.

Related Products: ABIN968535, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer SDS-PAGE buffer (62 mM Tris pH 6.8, 2 % SDS, 0.9 % b-mercaptoethanol, 0.003 % bromophenol blue, 5 % glycerol)
Storage 4 °C,-20 °C
Storage Comment Store the antibody at 4°C. Store the HeLa control lysate at -20°C.
Supplier Images
Western Blotting (WB) image for anti-P21-Activated Kinase 4 (PAK4) antibody (ABIN967342) Western blot analysis of PAK4. Lysate from HeLa cells was probed with anti-PAK4 (clon...
Product cited in: Knaus, Morris, Dong et al.: "Regulation of human leukocyte p21-activated kinases through G protein--coupled receptors." in: Science (New York, N.Y.), Vol. 269, Issue 5221, pp. 221-3, 1995 (PubMed).

Background publications Abo, Qu, Cammarano et al.: "PAK4, a novel effector for Cdc42Hs, is implicated in the reorganization of the actin cytoskeleton and in the formation of filopodia." in: The EMBO journal, Vol. 17, Issue 22, pp. 6527-40, 1999 (PubMed).

Validation Images
Catalog No. ABIN967342
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